Sequences from confirmation amplicon


Confirmation sequences for line 732B09 and hit At3g19590 are shown.
Line ID 732B09
Locus At3g19590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6805539
Primers used for PCR NJ72 - 8474, show primer details
   
Sequence Name 81-K036620-022-732-B09C-8409
GenBank Accession LN514023 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K036620-022-732-B09C-8409
CATTTACATTGAATAATCATTGGCCAGACATCTAACGGCCCATCTTCGTTGAACTCGTGT
TTTACATGATTCATTTTGGATCATTGAAGTTTCGACCATTTCGCTAATTCGAATTCAAAT
AAAATAAAATTAGTTTTTCTGTTTGGATATGTAGAGTAGATCTAATCGACCCTTGTACTT
CTGCAATTTGAATTCAAAATTTAAACCACTCTACTTTTGAATCTCTCTCTCACTAAACCT
AGAAAGTAGAAAACCCTAGCATTTGTGATCTTCAACGGGAAAAATGACGACTGTGACTCC
GTCCGCCGGTCGTGAGCTCTCGAATCCGCCGTCCGACGGCATTTCTAATCTCCGATTTTC
TAATAACAGTGATCATCTCCTCGTTTCTTCATGGGATAAGGTTAGTGAAACACCTCATTT
GCTCTTTGACTGATTTGATTCGAGTCCTCTCTTTCCTTCATCTGAAGTTTTTTTTTCTGT
TGTTCTGCGTTTGTTGAATCTAAGCGTGTGAGATTGTATGAGGTGAGCAC
   
Sequence Name 82-K036620-022-732-B09C-NJ72
GenBank Accession LN514145 [GenBank]
Sequencing primer gene specific primer, NJ72: 5'-GTGCTCACATCATACAATCTCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K036620-022-732-B09C-NJ72
CGCAGACACAGAGAAAAAAACTTCAGATGAAGGAAAGAGAGGACTCGAATCAAATCAGTC
AAAGAGCAAATGAGGTGTTTCACTAACCTTATCCCATGAAGAAACGAGGAGATGATCACT
GTTATTAGAAAATCGGAGATTAGAAATGCCGTCGGACGGCGGATTCGAGAGCTCACGACC
GGCGGACGGAGTCACAGTCGTCATTTTTCCCGTTGAAGATCACAAATGCTAGGGTTTTCT
ACTTTCTAGGTTTAGTGAGAGAGAGATTCAAAAGTAGAGTGGTTTAAATTTTGAATTCAA
ATTGCAGAAGTACAAGGGTCGATTAGATCTACTCTACATATCCAAACAGAAAAACTAATT
TTATTTTATTTGAATTCGAATTAGCGAAATGGTCGAAACTTCAATGATCCAAAATGAATC
ATGTAAAACACGAGTTCAACGAAGATGGGCCGTTAGATGTCTGGCCAATGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
CATTCA

Last Updated on 06.12.2018 16:11