Sequences from confirmation amplicon


Confirmation sequences for line 732E02 and hit At5g49950 are shown.
Line ID 732E02
Locus At5g49950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20320416
Primers used for PCR 18hg - 8474, show primer details
   
Sequence Name 32-K112479-0022-732-E02C-18hg
GenBank Accession LN500646 [GenBank]
Sequencing primer gene specific primer, 18hg: 5'-TCGGTTCTCTTATGTTGTAGCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K112479-0022-732-E02C-18hg
TTAGCTGTTAGTAATGCCACCTCACCTCACTAAAATTCAGAAGTCATATAGAGGGAACAA
TAGTCTGAATAGGGTATTTAACAGGATCCGAGTATTTGGGGAATTGGTTTTGATATGGGA
TAGTAGGAGATGCTCTAGAAAGTCATTCTTATCTGGTTTTGTTGTTGGTTTTGTTCTTGG
GTGCTCTAGAAGTTTTCACAAGATTACGGTTGCTAAATTGAGTTGGTAATTCTTCTGTTA
AACTGACTTGGTAGTTTCACTGTCATATTGAGTTTCCATGCATGGCTAAATTAAGAAGTG
AACGGCTTTAGGCATCGTTGGCGAAAAATTTGATGCGATTCTTGGTTGTATCTTTCTGCA
TTGCATAGTGATGGGTCTCCCTGTATTATGAAACTGATGTTCCAATGTCTCAACTTTTGC
AGGGCAAACAAAAACATAGTTCTGGCTACCACAACACATGGTGGTCATCTAGCTTACTAT
GAAGGGTTGACAGCGTCAAGCATGTGGTAATCAATCTGCGTAAATCCGCAGTCTTAGAAA
CTCATCTCACATTTCTATTCACAAGGACTTTCTTAGGAGTGAAAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCAGCGTTATTA
   
Sequence Name 02-K112478-0022-732-E02C-8409
GenBank Accession LN487101 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K112478-0022-732-E02C-8409
TTTTTCCTCCTAAGAAGTCCTTGTGAATAGAAATGTGAGATGAGTTTCTATGACTGCGGA
TTTACGCAGATTGATTACCACATGCTTGACGCTGTCAACCCTTCATAGTAAGCTAGATGA
CCACCATGTGTTGTGGTAGCCAGAACTATGTTTTTGTTTGCCCTGCAAAAGTTGAGACAT
TGGAACATCAGTTTCATAATACAGGGAGACCCATCACTATGCAATGCAGAAAGATACAAC
CAAGAATCGCATCAAATTTTTCGCCAACGATGCCTAAAGCCGTTCACTTCTTAATTTAGC
CATGCATGGAAACTCAATATGACAGTGAAACTACCAAGTCAGTTTAACAGAAGAATTACC
AACTCAATTTAGCAACCGTAATCTTGTGAAAACTTCTAGAGCACCCAAGAACAAAACCAA
CAACAAAACCAGATAAGAATGACTTTCTAGAGCATCTCCTACTATCCCATATCAAAACCA
ATTCCCCAAATACTCGGATCCTGTTAAATACCCTATTCAGACTATTGTTCCCTCTATCTA
ACTTCTGAATTTTAGTGAGGTGACGTGGCATTACTTAACAGCTAAATGTACTCCTTCTGA
GGACTTATCAGTGGCCCTACAAGGCTACAACATAAGAGAACCG

Last Updated on 06.12.2018 16:11