Sequences from confirmation amplicon


Confirmation sequences for line 733C02 and hit At4g21030 are shown.
Line ID 733C02
Locus At4g21030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11231722
Primers used for PCR MF14 - 8474, show primer details
   
Sequence Name 77-K031693-022-733-C02C-8409
GenBank Accession LN513483 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K031693-022-733-C02C-8409
TTACAATTGAAATTTTTTGAAGGTTGATGATTGCCTTGTTGGATCTCAGCCAAGATCATT
TGAGAAACCGAAGATTGATCTATCTTCGGAAGATTGGATTTATCACATATTCCAGTGACT
GGTATGTTCCTTAAAACTCCACCATGAGTCCAACATCTACAACAATTCTTGCATTTGTAG
CGCGGTTGAGACTTCTTGTTGTTGTTGAAGTAGCTGAATCTGGTTTGATCGGAATAACAC
CTTGGACACACTCGCGGTGGGGGCATCACATTCATTTCATTGTCTTCATTTGTAAAAACA
TTCAAATTATTCATTTCTCTCGAGAGGTTGTTTATTTTAATTTCTCCAAAAACGCGAAGA
CTTAATTTATAAGATCTTCTTGTTACTATGATCTTTTCTGTTGTGGAAAGTG
   
Sequence Name 29-K031693-022-733-C02C-MF14
GenBank Accession LN499170 [GenBank]
Sequencing primer gene specific primer, MF14: 5'-CACTTTCCACAACAGAAAAGATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K031693-022-733-C02C-MF14
GTCTTCGCGTTTTTGGAGAAATTAAAATAAACAACCTCTCGAGAGAAATGAATAATTTGA
ATGTTTTTACAAATGAAGACAATGAAATGAATGTGATGCCCCCACCGCGAGTGTGTCCAA
GGTGTTATTCCGATCAAACCAGATTCAGCTACTTCAACAACAACAAGAAGTCTCAACCGC
GCTACAAATGCAAGAATTGTTGTAGATGTTGGACTCATGGTGGAGTTTTAAGGAACATAC
CAGTCACTGGAATATGTGATAAATCCAATCTTCCGAAGATAGATCAATCTTCGGTTTCTC
AAATGATCTTGGCTGAGATCCAACAAGGCAATCATCAACCTTTCAAAAAATTTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
CACTCCCAAATGG

Last Updated on 06.12.2018 16:11