Sequences from confirmation amplicon


Confirmation sequences for line 733H02 and hit At4g03540 are shown.
Line ID 733H02
Locus At4g03540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1570787
Primers used for PCR 19aq - 8474, show primer details
   
Sequence Name 47-K118761-0022-733-H02C-19aq
GenBank Accession LN506343 [GenBank]
Sequencing primer gene specific primer, 19aq: 5'-AGAGAGCTTCCTTCTTAGCCATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118761-0022-733-H02C-19aq
GTATGTACAAAATTTCTTCTTATTTTCTTGTTGAGTCATTGTATGTAAATTATACATCTA
TGAGTTATGCGAATATGTGGTAACAGAATAAAACATGTTTGGCAGGTATTTTGTAATAGC
AAACGCTGTTGTGAGTGTTTACAGCTTTCTAGTTCTGTTTCTTCCAAAGGAGAGTTTGCT
TTGGAAATTCGTCGTCGTCTTGGATTTGGTACCAATTTTTATTCTCTGCATGCATTCATT
TGCATAATATATTATAAGTTCCCTTGGTTATTATAAAGTGATGTTGTTACTTATACCTGG
TTAATATATATTAACAACTGATCCTAAATATTATTTAAAAAGATTTTTTGTTTTTCATCG
CATATTAACAACATTATTTATTGGCAACAAAAATGCAATGAAATTAGTATTTTGTAGCAA
CATTTTCACAACTATTTTGCAGCATGTAGATTGTGATTTTCTCGTTATACTAAGTTTTAA
GTGGTGTAATATAAGAATTGAAAATTATGATGTTACTAATTTGTGTTATTCTTATAATGA
TCATTAGCTCAATTTGTGTTAAATATCCACCTGACTTTTTATTCGAGTTCATCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 22-K118760-0022-733-H02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118760-0022-733-H02C-8409
GCCATTTACATTGAATATATCCTGGATGAACTCGAATAAAAAGTCAGGGTGGGATATTTA
ACACNAAATTGGAGCTAATGGGATCATTATAAGAAATAACACANNAATTAGTAACNNATC
ATAATTTTCAANTNNTCTTATATTACACCANNCTTAAAACTNNTAGTATAACGAAGAAAA
TCACNAATCTACATGGGCTGCAAAATAGNTNNTGTGAAAANNTGTTGCTACAANAATACT
ANNATTTCATTGGGCATTTTTGTTGCCANATAAATAATGGGTTGTTAATNNATGCGAATG
AAAAACAAANNAAATCTTTNNTTAAATAATATTTAGGGAATCAGTNNTGTTAATATANNT
ATTAACCAGGGGTATAAGTNNAACAACATCACNNTTTATANNATAACCAAGGGGGGAACT
TNNATAATATANNTTATGCNNAAATGAANTGGGCATGCAGAAAAGAATAANNAAATTGGG
GTACCANNAATCCAAGAAACGAACGAACGAAAATTTCCANNAAGCANNAACTCTCCNNNT
TTGGAAGAAAAACAGAAAACTA

Last Updated on 06.12.2018 16:11