Sequences from confirmation amplicon


Confirmation sequences for line 734D10 and hit At5g62620 are shown.
Line ID 734D10
Locus At5g62620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25138376
Primers used for PCR MK13 - 8474, show primer details
   
Sequence Name 35-K030758-022-734-D10C-8409
GenBank Accession LN501710 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K030758-022-734-D10C-8409
CATTTACAATTGAATATATCCATTTACAAGTGATTGGTGTGAACTGACGGTTGTCTATGT
GCGTGGTTTCTTGGCAATATATGAAGGGATTTACGCTTGAGGATGCTACTGGTCTAACCA
TTAACGGGGACATAGATGTTCACTCTGTTTTCGCTGGCTCTCTCCCAACCTCGCATCCTA
GTTTTTCTCCTCAGAGGCATCTTGAGCTCTCGAGCAATTGGCAAGCCCCATCACTTCCTG
ATGAGCAAGTTGATATGTTCATTGGTATCCTTTCTGCTGGTAACCATTTTGCTGAGAGGA
TGGCTGTGAGGAGGTCGTGGATGCAACATAAACTCGTTAAATCTTCCAAAGTAGTGGCTC
GGTTCTTTGTTGCACTGGTAAGTTTAGACTAATTTACAAGTGATCTGATCTTGGTCTTCT
AGGTTAAAATGTAAGAGCTTCTCATATCGAATTGCAAGCTAAACTCAGATGTTTATTTGG
GCAGCACTCAAGGAAAGAAGTAAATGTGGAGCTAAAGAAGGAAGCTGAATTCTTTGGGGA
CATAGTTATAGTCCCTTACATGGACAGTTATGACCTTGTCGTCCTCAAAACCGTTGCAAT
TTGCGAGTACGGGGTGAGTCCCGCTGATTCAAAGATTCAACACTTGATGTTGTGTTTTCA
ATTATTCAATATAAGGTAAAGAGTTTCTCTATTCTCTGTTTTGCAGGCTCATCAACTTGC
AGCTAAATTCATCATGAAGTGTGATGACGATACATTTGTACAAGTGGATGCGGTTCTTAG
TGAAG
   
Sequence Name 74-K030758-022-734-D10C-MK13
GenBank Accession LN512993 [GenBank]
Sequencing primer gene specific primer, MK13: 5'-GGGTGTTTTCTTTGCTTCACTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K030758-022-734-D10C-MK13
ATGTATCGTCATCACACTTCATGATGAATTTAGCTGCAAGTTGATGAGCCTGCAACACAT
AGAATAGAGAAACTCTTTACCTTATATTGAATAATTGAAAACACAACATCAAGTGTTGAA
TCTTTGAATCAGCGGGACTCACCCCGTACTCGCAAATTGCAACGGTTTTGAGGACGACAA
GGTCATAACTGTCCATGTAAGGGACTATAACTATGTCCCCAAAGAATTCAGCTTCCTTCT
TTAGCTCCACATTTACTTCTTTCCTTGAGTGCTGCCCAAATAAACATCTGAGTTTAGCTT
GCAATTCGATATGAGAAGCTCTTACATTTTAACCTAGAAGACCAAGATCAGATCACTTGT
AAATTAGTCTAAACTTACCAGTGCAACAAAGAACCGAGCCACTACTTTGGAAGATTTAAC
GAGTTTATGTTGCATCCACGACCTCCTCACAGCCATCCTCTCAGCAAAATGGTTACCAGC
AGAAAGGATACCAATGAACATATCAACTTGCTCATCAGGAAGTGATGGGGCTTGCCAATT
GCTCGAGAGCTCAAGATGCCTCTGAGGAGAAAAACTAGGATGCGAGGTTGGGAGAGAGCC
AGCGAAAACAGAGTGAACATCTATGTCCCCGTTAATGGTTAGACCAGTAGCATCCTCAAG
CGTAAATCCCTTCATATATTGCCAAGAAACCACGCACATAGACAACCATCAGTTCACACC
AATCACTTGTAAATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
Primers used for PCR MK12 - 8474, show primer details
   
Sequence Name 77-K030759-022-734-D10C-MK12
GenBank Accession LN513479 [GenBank]
Sequencing primer gene specific primer, MK12: 5'-GAAGAAGAAGATTGAGGAACATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K030759-022-734-D10C-MK12
TTTCTTACCGGGGCTGATCTTTTGAACGTGGGAATATCATGGAGCTTCCACTGTGGTTTA
ACTCTTGGGTCACATATTACAGTGGTTGGGAAGCCACGAGCTGCTCATTCGGAGAAGGAC
CCTAAGATATCGATGTTAAAGGAAGGAGATGAAGCTGTGAAGGTTTCACAGTTTAAGTTG
GAGCTTCAGGGTTTGAAAGCAGTGGAAGGAGAAGAGCCACCTCGGATACTCCACTTGAAT
CCAAGGCTTAAGGGTGATTGGAGTGGTAAGCCTGTGATTGAGCAGAACACTTGCTATAGA
ATGCAATGGGGCTCAGCACAAAGATGTGAAGGATGGAGATCTAGGGATGATGAAGAGACT
GGTTAGTGTAGATATGTTCAAAAGCATTTATCTTTCATCTGTTGTTTTTCTTGGTGTTTT
TGTTGGGGATACTAATTAGTAAGAATGGTTGTAGTTGATGGTCAGGTTAAGTGCGAGAAA
TGGGCTCGGGATGATAGCATTACATCTAAAGAAGAAGAGTCTAGCAAGGCGGCTTCATGG
TGGCTTAGTCGATTAATAGGTCGGAGCAAGAAAGTAACTGTTGAATGGCCATTTCCATTC
ACAGTTGACAAGCTTTTCGTGCTTACTCTTAGTGCTGGATTGGAAGGCTACCATGTTAGT
GTCGATGGGAAGCATGTCACTTCCTTTCCATACCGAACTGTAAGTGATGTTTTGCCGTTT
ACCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
   
Sequence Name 38-K030759-022-734-D10C-8409
GenBank Accession LN502873 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K030759-022-734-D10C-8409
TTTACATTGAATATATCCTGATGGTAAACGGCAAAACATCACTTACAGTTCGGTATGGAA
AGGAAGTGACATGCTTCCCATCGACACTAACATGGTAGCCTTCCAATCCAGCACTAAGAG
TAAGCACGAAAAGCTTGTCAACTGTGAATGGAAATGGCCATTCAACAGTTACTTTCTTGC
TCCGACCTATTAATCGACTAAGCCACCATGAAGCCGCCTTGCTAGACTCTTCTTCTTTAG
ATGTAATGCTATCATCCCGAGCCCATTTCTCGCACTTAACCTGACCATCAACTACAACCA
TTCTTACTAATTAGTATCCCCAACAAAAACACCAAGAAAAACAACAGATGAAAGATAAAT
GCTTTTGAACATATCTACACTAACCAGTCTCTTCATCATCCCTAGATCTCCATCCTTCAC
ATCTTTGTGCTGAGCCCCATTGCATTCTATAGCAAGTGTTCTGCTCAATCACAGGCTTAC
CACTCCAATCACCCTTAAGCCTTGGATTCAAGTGGAGTATCCGAGGTGGCTCTTCTCCTT
CCACTGCTTTCAAACCCTGAAGCTCCAACTTAAACTGTGAAACCTTCACAGCTTCATCTC
CTTCCTTTAACATCGATATCTTAGGGTCCTTCTCCGAATGAGCAGCTCGTGGCTTCCCAA
CCACTGTAATATGTGACCCAAGAGTTAAACCACATGGAAGCTCCATGATATTCCCACGTT
TCAAAAGATCAGACCCGGTTAAGGAAACAGAGAGAGAACACGAGTTTGTCCCATGTTCCT
CA

Last Updated on 06.12.2018 16:11