Sequences from confirmation amplicon


Confirmation sequences for line 735A08 and hit At3g50130 are shown.
Line ID 735A08
Locus At3g50130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18591173
Primers used for PCR NL49 - 8474, show primer details
   
Sequence Name 11-K103979-0022-735-A08C-NL49
GenBank Accession LN491085 [GenBank]
Sequencing primer gene specific primer, NL49: 5'-GTTGCTGCTCAAATCTATAGGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K103979-0022-735-A08C-NL49
TTCATGGCGTCATATACATTTCAATGTCGTGTTTGGTACGTGCCATGACCATATTGACTG
CACGCCACTTGTGACGGTCCATGGGTAGGAGATGTTTGTTGCCATGGTGGAACGGACCAA
GTGAGACAGTCTGAGGGAAATAGGACTTCTTGTTGTTCTCTTGAAGGTATTGTGGAACTC
TATAGATACATAGTTTGTCCCAACTAGTTGTGGCGTCTTCTCTAAGTGCTTGCTCCATTT
TGTCTCTAATTGAGATCACCCATTCTTCTCGTGTCTCTTCGGGTTTTTGGTTTTGTTTTT
GGAGTTTTTGGCGCATCATGAGGGTGAGAAAGTACCATGTGAACATGTCTTTCTGGATCG
TAGACGGTTGTGGTCGATTAGTTCTTTTTCTTTTGACAGAATACCGCCAAGGAAGAATCG
GTCCAAATGGTCGTGCTGGCGGTAGCGGTGGTGGTGGTGGTGGTAGACTTTTGCTTTTCT
TTTTGAAGAAATGCCGCCGAGGAGGAATTGATCTAAAAGATGGTGGTGGTGGTGGACGAG
GAATTGATCTAAACGATGGTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTNCATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 27-K103980-0022-735-A08C-8409
GenBank Accession LN498356 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K103980-0022-735-A08C-8409
GAGCCTTTACATTGAATACATCGTTTAGATCAATTCCTCGTCCACCACCACCACCATCTT
TTAGATCAATTCCTCCTCGGCGGCATTTCTTCAAAAAGAAAAGCAAAAGTCTACCACCAC
CACCACCACCGCTACCGCCAGCACGACCATTTGGACCGATTCTTCCTTGGCGGTATTCTG
TCAAAAGAAAAAGAACTAATCGACCACAACCGTCTACGATCCAGAAAGACATGTTCACAT
GGTACTTTCTCACCCTCATGATGCGCCAAAAACTCCAAAAACAAAACCAAAAACCCGAAG
AGACACGAGAAGAATGGGTGATCTCAATTAGAGACAAAATGGAGCAAGCACTTAGAGAAG
ACGCCACAACTAGTTGGGACAAACTATGTATCTATAGAGTTCCACAATACCTTCAAGAGA
ACAACAAGAAGTCCTATTTCCCTCAGACTGTCTCACTTGGTCCGTTCCACCATGGCAACA
AACATCTCCTACCCATGGACCGTCACAAGTGGCGTGCAGTCAATATGGTCATGGCACGTA
CCAAACACGACATTGAAATGTATATTGACGCCATGAAAGAACTCGAAGACAGGGCTCGTG
CTTGCTATGAAGGTCCTATAG

Last Updated on 06.12.2018 16:11