Sequences from confirmation amplicon


Confirmation sequences for line 735D06 and hit At2g45850 are shown.
Line ID 735D06
Locus At2g45850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18872005
Primers used for PCR 14um - 8474, show primer details
   
Sequence Name 55-K117671-0022-735-D06C-14um
GenBank Accession LN508927 [GenBank]
Sequencing primer gene specific primer, 14um: 5'-AATCAGAATTGCAAGCAAATTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K117671-0022-735-D06C-14um
AAGAAGGAACTCTGCAAATCTTCAGAGTCACAGAAACAGTAAAACCAAACAAGCTTAGAG
ATATGAGAGGTACACTAATAGAGTTAATCTTTTTCCCTAACATTTCCACATAATCTTTTT
CCCTAACATTTCCACATACTTTTGTTCAGAGGTTAGCTTATATTCCTTCTTCTTCTTCCT
AGATTCCATGCTTTATATACACAGAGTTCTTTCTCGCTATCATCAACCGCGCATTAAATC
AATATCAGCATGAGCATGACGCATATCCATTTGCCTTGAACCTGTTGACCAAATCAGGTT
TTGGTTTGGCTGCTGCTGAGGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 07-K117670-0022-735-D06C-8409
GenBank Accession LN489455 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K117670-0022-735-D06C-8409
CATTTACAATTGAATATTCCTGTCCCTCAGCAGCAGCCAAACCAAAACCTGATTTGGTCA
ACAGGTTCAAGGCAAATGGATATGCGTCATGCTCATGCTGATATTGATTTAATGCGCGGT
TGATGATAGCGAGAAAGAACTCTGTGTATATAAAGCATGGAATCTAGGAAGAAGAAGAAG
GAATATAAGCTAACCTCTGAACAAAAGTATGTGGAAATGTTAGGGAAAAAGATTATGTGG
AAATGTTAGGGAAAAAGATTAACTCTATTAGTGTACCTCTCATATCTCTAAGCTTGTTTG
GTTTTACTGTTTCTGTGACTCTGAAGATTTGCAGAGTTCCTTTCTTTCTCTGTTTTAGAT
TGTTCAGTCTTTATGTAATTTGCTTGCAATTCTGATT

Last Updated on 06.12.2018 16:11