Sequences from confirmation amplicon


Confirmation sequences for line 735H09 and hit At3g60640 are shown.
Line ID 735H09
Locus At3g60640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22416150
Primers used for PCR LD14 - 8474, show primer details
   
Sequence Name 11-K030599-022-735-H09C-8409
GenBank Accession LN490989 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K030599-022-735-H09C-8409
TTTCGGTATAATAAAATCCTTCTTCTTCTTTTTTTTCCAGTAATTTAAGTTAGTATCGAT
CGGTCGAAGATCGATTTATATTCGCAGAGAAGAGGAAAGCTGAGGCTTTAAGGATCAGAG
AGAAGTATTCTGACAGAGTCCCGGTATTCTTACTCTCTTCTTATGTCACTGCCATCGATT
TTGTTCTGTTTCCTAGTTTTCCTGATATTGCTCACATAGGAATATACCCTATTTGAGAAG
CTCTTGTGAGGCCTGATTGATTGAAATCTTGCAAAAATAACACTTTTTGATAGATAGATG
TGGTTCTTGTTGAGAGTTCAATGCATAGTTTCAGAATAAGATGGTTATATTAATGTCTCT
CTTTTGTAAT
   
Sequence Name 50-K030599-022-735-H09C-LD14
GenBank Accession LN507060 [GenBank]
Sequencing primer gene specific primer, LD14: 5'-GGGAGGAAGAACATTATCAACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K030599-022-735-H09C-LD14
AATCACATACACAAACGGCCTACCGTTAGATCAGCTGGGACAAGGTATCTGTAAAACGTA
AAATCCAAAACATGAAAATTAAGCAAGAGAGACTCCAACGTTAAGAAATCAGATTTGATA
TTATAAAGAAAAGAGGATCATACTTCTTCTTGTCAATGTTTGGGATATCACTTTTCTCTG
ACTTCTCCACAATCACCTATTACAAAAGAGAGACATTAATATAACCATCTTATTCTGAAA
CTATGCATTGAACTCTCAACAAGAACCACATCTATCTATCAAAAAGTGTTATTTTTGCAA
GATTTCAATCAATCAGGCCTCACAAGAGCTTCTCAAATAGGGTATATTCCTATGTGAGCA
ATATCAGGAAAACTAGGAAACAGAACAAAATCGATGGCAGTGACATAAGAAGAGAAGTAG
AATACCGGGACTCTGTCAGAATACTTCTCTCT

Last Updated on 06.12.2018 16:11