Sequences from confirmation amplicon


Confirmation sequences for line 736A02 and hit At3g50910 are shown.
Line ID 736A02
Locus At3g50910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18921522
Primers used for PCR 57sm - 8474, show primer details
   
Sequence Name 51-K116491-0022-736-A02C-57sm
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 57sm: 5'-CCTCTGGTATCAACAGAGTTGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K116491-0022-736-A02C-57sm
GAGTATGATGGTCCATATGGAGAAACTTAAAGAGGTATTTACCCCTTTTTTTTCCCTTC
   
Sequence Name 19-K116490-0022-736-A02C-8409
GenBank Accession LN494918 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116490-0022-736-A02C-8409
CTACCGCCTCCCCAACAACATGAATCTATCAGAAGAACTACGCTGTCAACTCGAGATTGC
GAGGTTTGTCTCTGACTCTTTAGGCAGAGGTATGGCAAAGGCCGAGGTAGAGATGGAGAT
GGAATCTATGCTCGAAACTAAGAACTTTGAAATCACACGGTTGTCTGACCGTCTACACTA
CTACGAGGCCGTGAACAGAGAGATGTCTCAACGAAACCAAGAAGCCATAGGTAGTAGAAA
ATCACTCTCATTCACCAAATTTATCCATTGTCATTTTCTATCGTATAATAAAAATCTAAT
CTCACAACTCTGTTGATACCAGAGG

Last Updated on 06.12.2018 16:11