Sequences from confirmation amplicon


Confirmation sequences for line 736A08 and hit At3g63420 are shown.
Line ID 736A08
Locus At3g63420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23417239
Primers used for PCR MA61 - 8474, show primer details
   
Sequence Name 74-K102435-0022-736-A08C-8409
GenBank Accession LN513047 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K102435-0022-736-A08C-8409
ATTTACAATTGAATATATCCTGCAATACTATTATTTGGGCCAATTCTTGCAAATGAGCCC
ATGGGCTCAACAAAGGGATATGTGTTGTCGCAACAAACAACGTGACCGTACATAAACATG
TGGTTGGAGTCAGTACTGACTCTGTTAGAGTTAGAGGGTTTAGTCACACTCTCATTCTCG
TTTGTGTTTTTGATTGGCGGAGAATTGGTGATAGATAAGCTTCTTCTTCCCTCTTCTCAA
CTTGGTGGATCTGTCATCGATGGCCTCTTTGATGCTCAACGGGGCCATTACCTTCCCCAA
AGGTTTAGGTTCCCCTGGTTCCAATTTGCATGCCAGATCGATTCCTCGGCCGACCTTACT
CTCAGTTACCCGAACCTCCACACCTAGACTCTCGGTGGCTACTAGATGCAGCAGCAGCAG
CGTGTCGTCTTCCCGGCCATCGGCGCAACCTAGGTTCATTCAGCACAAGAAGGAGGCTTA
CTGGTTCTACAGGTTCTTATCCATCGTATACGACCATGTCATCAATCCTGGGCATTGGAC
CGAGGATATGAGAGACGACGCTCTTGAGCCAGCGGATCTCAGCCATCCGGACATGCGAGT
GGTCGATGTCGGCGGCGGAACTGGTTTCACTAC
   
Sequence Name 74-K102436-0022-736-A08C-MA61
GenBank Accession LN513048 [GenBank]
Sequencing primer gene specific primer, MA61: 5'-GACTATGCCCAGAGTAGTGAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K102436-0022-736-A08C-MA61
CCGGAGGCTGAGACCGCTGGCTCAGAGCGTCGTCTCTCATATCCTCGGTCCAATGCCCAG
GATTGATGACATGGTCGTATACGATGGATAAGAACCTGTAGAACCAGTAAGCCTCCTTCT
TGTGCTGAATGAACCTAGGTTGCGCCGATGGCCGGGAAGACGACACGCTGCTGCTGCTGC
ATCTAGTAGCCACCGAGAGTCTAGGTGTGGAGGTTCGGGTAACTGAGAGTAAGGTCGGCC
GAGGAATCGATCTGGCATGCAAATTGGAACCAGGGGAACCTAAACCTTTGGGGAAGGTAA
TGGCCCCGTTGAGCATCAAAGAGGCCATCGATGACAGATCCACCAAGTTGAGAAGAGGGA
AGAAGAAGCTTATCTATCACCAATTCTCCGCCAATCAAAAACACAAACGAGAATGAGAGT
GTGACTAAACCCTCTAACTCTAACAGAGTCAGTACTGACTCCAACCACATGTTTATGTAC
GGTCACGTTGTTTGTTGCGACAACACATATCCCTTTGTTGAGCCCATGGGCTCATTTGCA
AGAATTGGCCCAAATAATAGTATTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11