Sequences from confirmation amplicon


Confirmation sequences for line 736C12 and hit At4g15900 are shown.
Line ID 736C12
Locus At4g15900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9026560
Primers used for PCR 20jr - 8474, show primer details
   
Sequence Name 50-K113214-0022-736-C12C-8409
GenBank Accession LN507229 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K113214-0022-736-C12C-8409
TTAATTGCTTCTGCATCAGCTGACAACACCAAGAAATTTAGCCTTCCAAAGGGAGAGTTC
TGCCACAACATGCTGTAAGTCTCTGCTAGATAATTATATACTTTGCATCTCCAGTGCTTT
TGAAATAGGAGCTGTCATTATTAATTATGTAATTGATGTGTTTGAACAATGTTTATCTTT
TTCCATGGAAAAGTTCGCAACAGAAAACCATAATTAATGCAATGGCTGTAAACGAGGATG
GTGTGATGGTCACTGGAGGTATATAATCATTTCTGATTCCTTTCGTCCCTGTGATTGTTT
TCCTTTGATAGTAAAATGGGTGAGACATAATCATTTTCAACTGAAACTGATTCTATATAT
GTGTGAATGAAGGTGATAATGGAAGTATATGGTTCTGGGACTGGAAGAGTGGTCACAGTT
TCCAACAGTCAGAAACTATCGTACAGCCTGGTACGTACATTTATAGCTTTCATGGTCTTT
TGCTCCTGATCTCCTTGTTA
   
Sequence Name 50-K113215-0022-736-C12C-20jr
GenBank Accession LN507230 [GenBank]
Sequencing primer gene specific primer, 20jr: 5'-ATAACAAGGAGATCAGGAGCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K113215-0022-736-C12C-20jr
GATAGTTTCTGACTGTTGGAAACTGTGACCACTCTTCCAGTCCCAGAACCATATACTTCC
ATTATCACCTTCATTCACACATATATAGAATCAGTTTCAGTTGAAAATGATTATGTCTCA
CCCATTTTACTATCAAAGGAAAACAATCACAGGGACGAAAGGAATCAGAAATGATTATAT
ACCTCCAGTGACCATCACACCATCCTCGTTTACAGCCATTGCATTAATTATGGTTTTCTG
TTGCGAACTTTTCCATGGAAAAAGATAAACATTGTTCAAACACATCAATTACATAATTAA
TAATGACAGCTCCTATTTCAAAAGCACTGGAGATGCAAAGTATATAATTATCTAGCAGAG
ACTTACAGCATGTTGTGGCAGAACTCTCCCTTTGGAAGGCTAAATTTCTTGGTGTTGTCA
GCTGATGCAGAAGCAATTAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCACGNTTATT

Last Updated on 06.12.2018 16:11