Sequences from confirmation amplicon


Confirmation sequences for line 737D02 and hit At3g26890 are shown.
Line ID 737D02
Locus At3g26890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9909960
Primers used for PCR 61ad - 8474, show primer details
   
Sequence Name 53-K106210-0022-737-D02C-61ad
GenBank Accession LN508163 [GenBank]
Sequencing primer gene specific primer, 61ad: 5'-GGAGTTCACTGATGATTCGTGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K106210-0022-737-D02C-61AD
TCAGTGCTCATGGATCGACTAGTTATGCTGCAGGTAAGGTTAGTTCGAGTCTTACTGGAG
CTAGAAGGATAGTTGGTTTTGCGTCAGGCGAAACAAGCTCACTGGACAACAAACAAACGA
GTGTCGCTGTTGATCACTCTCTATCATCCACTGTTGGAGTTGCAGGAGTAGATATCGGTG
GAGCATTAGTCAGGAAACGAGTACTCTCCCCCTTAAATACTCTTTTCCCTGTCAAATTCA
GAGGTGACCTGCATGATATCAGCTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCACGATTATTA
   
Sequence Name 69-K106211-0022-737-D02C-8409
GenBank Accession LN512211 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K106211-0022-737-D02C-8409
ATTTACGCTGATATCATGCAGGTCACCTCTGAATTTGACAGGGAACAGAGTATTTAAGGG
GGAGAGTACTCGTTTCCTGACTAATGCTCCACCGATATCTACTCCTGCAACTCCAACAGT
GGATGATAGAGAGTGATCAACAGCGACACTCGTTTGTTTGTTGTCCAGTGAGCTTGTTTC
GCCTGACGCAAAACCAACTATCCTTCTAGCTCCAGTAAGACTCGAACTAACCTTACCTGC
AGCATAACTAGTCGATCCATGAGCACTTGATGAGGTAACAACACAAGTCCCACGGAATCT
ACACGAATCATCANTGAACTC

Last Updated on 06.12.2018 16:11