Sequences from confirmation amplicon


Confirmation sequences for line 737E05 and hit At1g79440 are shown.
Line ID 737E05
Locus At1g79440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29886277
Primers used for PCR IF03 - 8474, show primer details
   
Sequence Name 13-K032517-022-737-E05C-8409
GenBank Accession LN491925 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K032517-022-737-E05C-8409
CCATTTACAATTGAATATAATATATCAATCAAAAACCACTCCAACACTACCAACTCAAGG
AAGAACTTGGACAACTTATAACTTTGGAGCAAGGAAAACCACTCAAGGAAGCCATCGGAG
AGGTTAATGATTCATTTTTTATTTGAGAACATTGAATGTGCTGTTTAGGCTTGTCTCAAC
TGTTGCAATCGTTTCTTAAATGCAATATATGATTCTGCAATTTAGTTTACAGGTAGCATA
TGGGGCAAGTTTTATTGAGTACTATGCGGAGGAGGCAAAACGTGTATATGGTGATATAAT
TCCTCCGAATCTGTCTGATCGCCGACTGTTGGTTCTAAAACAGGTTTCCCCATTCATGCT
TCTATATTTAATACATGCAGCTTTTTTGTAGTGACATAGTTCTGCAC
   
Sequence Name 13-K032517-022-737-E05C-IF03
GenBank Accession LN491926 [GenBank]
Sequencing primer gene specific primer, IF03: 5'-GGGTGCAGAACTATGTCACTACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K032517-022-737-E05C-IF03
GAATGGGGAACCTGTTTTAAACCAACAGTCGGCGATCAGACAGATTCGGAGGAATTATAT
CACCATATACACGTTTTGCCTCCTCCGCATAGTACTCAATAAAACTTGCCCCATATGCTA
CCTGTAAACTAAATTGCAGAATCATATATTGCATTTAAGAAACGATTGCAACAGTTGAGA
CAAGCCTAAACAGCACATTCAATGTTCTCAAATAAAAAATGAATCATTAACCTCTCCGAT
GGCTTCCTTGAGTGGTTTTCCTTGCTCCAAAGTTATAAGTTGTCCAAGTTCTTCCTTGAG
TTGGTAGTGTTGGAGTGGTTTTTGATTGATATATTTATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11