Sequences from confirmation amplicon


Confirmation sequences for line 737F10 and hit At2g24440 are shown.
Line ID 737F10
Locus At2g24440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10392000
Primers used for PCR NL56 - 8474, show primer details
   
Sequence Name 39-K104096-0022-737-F10C-NL56
GenBank Accession LN503333 [GenBank]
Sequencing primer gene specific primer, NL56: 5'-AAACGAATTGCTTCAAACTAGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K104096-0022-737-F10C-NL56
ATCCCATATATANTCAGGAAGTTTTAGCATAAAGAAACTGCACAAAGCCGATCAAGCACT
ACCAAACGACGAAACCGTGCTCAAATGGTTTTCACCAATATATCAAAATTAAGCCTAAAA
AATCCTGCAGTTGCTCTAGATAGTCAGATATTGAAGCCTATTAGCCGAGGGCCAAGGAGG
AAACGAAAGACTCGGCATTGGTAGAACCTACTAGCTGTGTTACTTAATCTTCTCTACGAT
GTCCGCAATGACCTCTTCCATGTTAAGTTCCTTCATCGGTGTAAATGGGCGCTTCATAGC
CTACATAAGAAAATCACATCATCTTCTATATACATCTGAGAATAAAGAACATGAAAGCAA
ACATTTTCTTATTGTACACCTAGTCACTAACAATCAGATCTGCTAGTTTGTTCTTATTTC
AAACAACTTAGCACTCAACTTGATTCAGTTAAAAAGCAAACAACCAAAGGCCATTTTATG
AATGCTCTAACAAAACCTCATTGGTGATCAGTTGACTCAGCAAAAATAGCCTCAATTTCG
TGTGAGCTTAAGAAAATAAAGTTGATTTTCACTTTTTAAAGTATACCATCAAAGAAACTA
TGTTATGCGAATAGATACAGACACGACGAGAAACATCAATCCAACTGGAACAAGTCACTC
ACATAAGTGCGACTAACTGGCATACTACATTCAAGTATGAGAGTGAAAAACTACATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAATGTA
   
Sequence Name 55-K104097-0022-737-F10C-8409
GenBank Accession LN508794 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K104097-0022-737-F10C-8409
GAAGCCATGTAGTTTTTCACTCTCATACTTGAATGTAGTATGCCAGTTAGTCGCACTTAT
GTGAGTGACTTGTTCCAGTTGGATTGATGTTTCTCGTCGTGTCTGTATCTATTCGCATAA
CATAGTTTCTTTGATGGTATACTTTAAAAAGTGAAAATCAACTTTATTTTCTTAAGCTCA
CACGAAATTGAGGCTATTTTTGCTGAGTCAACTGATCACCAATGAGGTTTTGTTAGAGCA
TTCATAAAATGGCCTTTGGTTGTTTGCTTTTTAACTGAATCAAGTTGAGTGCTAAGTTGT
TTGAAATAAGAACAAACTAGCAGATCTGATTGTTAGTGACTAGGTGTACAATAAGAAAAT
GTTTGCTTTCATGTTCTTTATTCTCAGATGTATATAGAAGATGATGTGATTTTCTTATGT
AGGCTATGAAGCGCCCATTTACACCGATGAAGGAACTTAACATGGAAGAGGTCATTGCGG
ACATCGTAGAGAAGATTAAGTAACACAGCTNGTAGGTTCTACCAATGCCGAGTCTTTCGT
TTCCTCCTTGGCCCTCGGCTAANAGGCTTCAATATCTGACTATCTNGAGCAACTGCAGGA
TTTTTTAGGCTTAATTTTGATATATTGGTGAAAACCATTTGAGCACGGTTTCGTCGTTTG
GTAGTGCTTGATCGGCTTTGTGCAGTTTCTTTATGCTAAAACTTCCTGATATATATGGGA
TTAAGTCTGTCTTCGTAATGC

Last Updated on 06.12.2018 16:11