Sequences from confirmation amplicon


Confirmation sequences for line 738D01 and hit At2g32680 are shown.
Line ID 738D01
Locus At2g32680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13862137
Primers used for PCR CR67 - 8474, show primer details
   
Sequence Name 10-K105749w-0022-738-D01C-CR67
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, CR67: 5'-ATTTTACTGGTATTGTGGCCTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K105749w-0022-738-D01C-CR67
GAACGAGTTTGACACCCGCGGTTGCAACAACAGTGACACCTTTAATGGAGTCTGGTGCGA
TAACTCGACGGGTGCGGTCGCAGTGCTACAACTAAGGAAGTGTCTCAGTGGAACTTTGAA
GTCCAACAGTAGCCTCTTCGGGTTTCATCAACTCCGTTACGTTGATCTCCAAAACAACAA
CTTAACCTCCTCTTCACTCCCTTCCGGGTTTGGAAATCTCAAAAGATTAGAGGGCTTGTT
TCTTTCCTCTAATGGCTTCCTCGGCCAAGTTCCTTCCTCATTCAGTAACCTAACAATGCT
TGCTCAATTAGACCTTTCTTATAACAAGCTCATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATACAAAAATGTAGATGTCCG
   
Sequence Name 50-K105750-0022-738-D01C-8409
GenBank Accession LN507170 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K105750-0022-738-D01C-8409
CGGACTGAGCATTTACATTGAATATGAGCTTGTTATAAGAAAGGTCTAATTGAGCAAGCA
TTGTTAGGTTACTGAATGAGGAAGGAACTTGGCCGAGGAAGCCATTAGAGGAAAGAAACA
AGCCCTCTAATCTTTTGAGATTTCCAAACCCGGAAGGGAGTGAAGAGGAGGTTAAGTTGT
TGTTTTGGAGATCAACGTAACGGAGTTGATGAAACCCGAAGAGGCTACTGTTGGACTTCA
AAGTTCCACTGAGACACTTCCTTAGTTGTAGCACTGCGACCGCACCCGTCGAGTTATCGC
ACCAGACTCCATTAAAGGTGTCACTGTTGTTGCAACCGCGGGTGTCAAACTCGTTCGTGA
ACTTCGTGAAGGCTTGAATCTGGTGGGGACGACAGGCCACAATACCAGTAAAA

Last Updated on 06.12.2018 16:11