Sequences from confirmation amplicon


Confirmation sequences for line 738F09 and hit At5g55720 are shown.
Line ID 738F09
Locus At5g55720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22556256
Primers used for PCR CR68 - 8474, show primer details
   
Sequence Name 81-K107250-0022-738-F09C-CR68
GenBank Accession LN514077 [GenBank]
Sequencing primer gene specific primer, CR68: 5'-CCCAATTCTTTCAATTTTCACCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K107250-0022-738-F09C-CR68
ATTTTTAGTGCCTTCATGTCTCTTGCATATATATATTGGAGCAAATTATAAACATAATGT
TCATAGTGAAATTGTTAGTTGGTTTTAATCTCTTTTTAAGACAACATATAATGTCAATTG
TATGTACGTTTTTCTTGTTTCTCTTAAACACTTCTTTTGCGTTTGCATTTGCGATTCCTA
AACCGCCAATAGTAAGGAGACTATCAACGACAGTGACATCAAATTCAACAGCGTCTTCTT
GCTCAGCCAATGGAAATCCAATCGATGAGTGTTGGAGATGCGACGAAAACTGGAAGGACA
ACCGCAAAAACCTCGCGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 65-K107249-0022-738-F09C-8409
GenBank Accession LN511412 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K107249-0022-738-F09C-8409
CGGACTGAGCCATTTACAATTGAATATATCCGCGAGGTTTTTGCGGTTGTCCTTCCAGTT
TTCGTCGCATCTCCAACACTCATCGATTGGATTTCCATTGGCTGAGCAAGAAGACGCTGT
TGAATTTGATGTCACTGTCGTTGATAGTCTCCTTACTATTGGCGGTTTAGGAATCGCAAA
TGCAAACGCAAAAGAAGTGTTTAAGAGAAACAAGAAAAACGTACATACAATTGACATTAT
ATGTTGTCTTAAAAAGAGATTAAAACCAACTAACAATTTCACTATGAACATTATGTTTAT
AATTTGCTCCAATATATATATGCAAGAGACATTGAAGTGCACTAAAAAATAAATACGGAG
GTAATTAAATTAGGTGAAAATTGAAAGAATTGGG

Last Updated on 06.12.2018 16:11