Sequences from confirmation amplicon


Confirmation sequences for line 739A08 and hit At5g67200 are shown.
Line ID 739A08
Locus At5g67200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26815557
Primers used for PCR EU52 - 8474, show primer details
   
Sequence Name 56-K103544-0022-739-A08C-EU52
GenBank Accession LN509074 [GenBank]
Sequencing primer gene specific primer, EU52: 5'-AACCCTTGAGATTGAAGAAGATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K103544-0022-739-A08C-EU52
TAGCTGCCATTAGGATGATAATCGTAGATGATAAGTCTCTCTCCGTTGGACTGAAAATAA
GATCTAATCGGAACAAGATTGGTATGTCTAAGTCCACCAACAATCTCCATATGATTCTCA
AACGCTTCTTCACTCGTCACTGCCGTTTTAGCAGCGTCTAGTCTCTTCACCGTCACAATA
AGCTGATTATCAAGAACCGCTTTATACGTAATCCCTACTGATCCTCTTCCTAATAACTCC
GCTGAAGCTCTCATTAGCTGCTCCATTGTGTACATTCCTTGACTTCTAGATTCTCCACAG
AAAACAAGATTCCCACTATTTGGTATTCGCTGCTCTGTTTCTTGAAATTGAACCTCTTTT
TCTCGCTTTTGAGATTCGGTATCTGAGTTCAAAACCGGAACTGCTCTTGTTCTTGGAGTT
TGGTCTTGTTCAAAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 72-K103545-0022-739-A08C-8409
GenBank Accession LN512709 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K103545-0022-739-A08C-8409
TCGGACATGAGCCTTTACAATTGAATATATCCTGTTTTGAACAAGACCAAACTCCAAGAA
CAAGAGCAGTTCCGGTTTTGAACTCAGATACCGAATCTCAAAAGCGAGAAAAAGAGGTTC
AATTTCAAGAAACAGAGCAGCGAATACCAAATAGTGGGAATCTTGTTTTCTGTGGAGAAT
CTAGAAGTCAAGGAATGTACACAATGGAGCAGCTAATGAGAGCTTCAGCGGAGTTATTAG
GAAGAGGATCAGTAGGGATTACGTATAAAGCGGTTCTTGATAATCAGCTTATTGTGACGG
TGAAGAGACTAGACGCTGCTAAAACGGCAGTGACGAGTGAAGAAGCGTTTGAGAATCATA
TGGAGATTGTTGGTGGACTTAGACATACCAATCTTGTTCCGATTAGATCTTATTTTCAGT
CCAACGGAGAGAGACTTATCATCTACGATTATCATCCTAATGGCAGCTTATTCAATCTCA
TTCACGGTAAGCTCTCTCATCTTCTTCAATCTCAAGGGT

Last Updated on 06.12.2018 16:11