Sequences from confirmation amplicon


Confirmation sequences for line 740A10 and hit At4g26860 are shown.
Line ID 740A10
Locus At4g26860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13504672
Primers used for PCR CR70 - 8474, show primer details
   
Sequence Name 14-K107367-0022-740-A10C-CR70
GenBank Accession LN492535 [GenBank]
Sequencing primer gene specific primer, CR70: 5'-TAATACCTTTTCTCCATCCACACC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K107367-0022-740-A10C-CR70
TGCATTTGGGACTCCGCTGCAACATTGGATTAAACGACTTTCACTTTCACTAAACCCCTA
ATTCAATATTAAAGATGGGGATAAAAGTCAGACTCAATAGCGTTTTGGCTTTGTTGCTCT
GTAAATGCCCCACGAAATGCCACTCTATATCTTCCGGAAGCTGTATATCAATCAAACAAA
GTGAAGGAGAAGCTCCATTAGCATCATCAAAAGCACAAAATATATCACATTCCCAGAAAA
TGGAGAGACACATAGTAGTTAGTGATTGAAGGATAAACATTCGGATTTTGAAATGAAATC
GATTATCTAGTGAAGATAACAAGGATTGGAGAGAGGAACCTGTGGAGCTTTATCGATGAT
TTCTTGAACGTAATTCTCGCCGAAGCAACGGTGACCTGCGTCGTAGATTTGGCGGATAAG
TGAGACGGGTTTAGTCTTGGAGACTGGAAGGACCCTGACCCGCTCGGGATCTCTTCCTAC
TTGCTCCGCCGCCTTACGAGCTCTAAGAATCACAGAGCGGAGAGCACTTGCGACGGTTGC
TTACCGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGC
   
Sequence Name 30-K107368-0022-740-A10C-8409
GenBank Accession LN499746 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K107368-0022-740-A10C-8409
ATTTACATTGAATATATCCTGCGGTAAGCAACCGTCGCAAGTGCTCTCCGCTCTGTGATT
CTTAGAGCTCGTAAGGCGGCGGAGCAAGTAGGAAGAGATCCCGAGCGGGTCAGGGTCCTT
CCAGTCTCCAAGACTAAACCCGTCTCACTTATCCGCCAAATCTACGACGCAGGTCACCGT
TGCTTCGGCGAGAATTACGTTCAAGAAATCATCGATAAAGCTCCACAGGTTCCTCTCTCC
AATCCTTGTTATCTTCACTAGATAATCGATTTCATTTCAAAATCCGAATGTTTATCCTTC
AATCACTAACTACTATGTGTCTCTCCATTTTCTGGGAATGTGATATATTTTGTGCTTTTG
ATGATGCTAATGGAGCTTCTCCTTCACTTTGTTTGATTGATATACAGCTTCCGGAAGATA
TAGAGTGGCATTTCGTGGGGCATTTACAGAGCAACAAAGCCAAAACGCTATTGAGTCTGA
CTTTTATCCCCATCTTTAATATTGAATTAGGGGTTTAGTGAAAGTGAAAGTCGTTTAATC
CAATGTTTGCAGCTGGAGTCCCAAATTTGGCAATGGTTCATGGTGTGGATGGAGAAAANG
TATTA

Last Updated on 06.12.2018 16:11