Sequences from confirmation amplicon


Confirmation sequences for line 740B06 and hit At4g39940 are shown.
Line ID 740B06
Locus At4g39940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18520976
Primers used for PCR 06mp - 8474, show primer details
   
Sequence Name 58-K114277-0022-740-B06C-06mp
GenBank Accession LN509773 [GenBank]
Sequencing primer gene specific primer, 06mp: 5'-TTGTTTTGCAGGTAAGAGATGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K114277-0022-740-B06C-06mp
CACTACCTGGAGGATATTACATCAATACTTTATTAGTACAAATTTACGTTCCAAAATCTA
TCTGATCATTCACGTGACGAACCTCGCAATTCACTGGTGCCTCGTAAGGGTCGTCGATTC
CAGTGAAGCCTGGATAATTAATTCAAGAAAACTCAACAATTAGCTAGGTCAATAAGTTAC
ACTCAACAATTAGGTCAATAAGTCAAACTCTACGAATCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 28-K114276-0022-740-B06C-8409
GenBank Accession LN498950 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K114276-0022-740-B06C-8409
TGACTTATTGACCTAATTGTTGAGTGTAACTTATTGACCTAGCTAATTGTTGAGTTTTCT
TGAATTAATTATCCAGGCTTCACTGGAATCGACGACCCTTACGAGGCACCAGTGAATTGC
GAGGTTCGTCACGTGAATGATCAGATAGATTTTGGAACGTAAATTTGTACTAATAAAGTA
TTGATGTAATATCCTCCAGGTAGTGCTGAAACACACAGGAGACGACGAGTCGTGTTCGCC
ACGTCAGATGGCTGAGAACATCATCTCTTACTGGCAAAACA

Last Updated on 06.12.2018 16:11