Sequences from confirmation amplicon


Confirmation sequences for line 740F11 and hit At2g42670 are shown.
Line ID 740F11
Locus At2g42670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17772500
Primers used for PCR 52fr - 8474, show primer details
   
Sequence Name 28-K111563-0022-740-F11C-52fr
GenBank Accession LN498905 [GenBank]
Sequencing primer gene specific primer, 52fr: 5'-CTGATTGGTCTTGAATGCTGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K111563-0022-740-F11C-52fr
ATGATTCAGATAAAAATAGCATAAGAGTCCCATTTTAACTTCCTAGGTTTAAGTAGCAAT
GGTTCTGCTTGGAATAATATGCAAATACCGTTCAAGGTTAAAACACTCTATAGCCAATCC
TGAGACAAAAATTTTAGTGGATATTTAATCTTCTCCTTTTCCGCATATTCAAAATTAGGG
GAATAGGGGGGCATCCAAACATGGTCCTTGAGAGAATCTCAGGAACCAACTATTCAAGCC
ATGAGCCTGAAGTCTGCAAAAAAGCCTATGACAAATGGTCTAGAAAGTATGAGTAAACAC
AGTTCGAGTCAATTCTGAGTCTGTCTCTAGTTGGGTGTTTTATCTTCCTGATACTGCCAC
CAATGTTATCTCTCACTTGGTTCATTTTGCCTCTGTTGTCAAAACAGATAGGAATCTTCT
GTATGCCACCTTCTTCTATGATACCTCTTCATAACCATCCGGGCATGACCGTGCTAAGCA
AGCTCGTTTATGGTTCAATGCATGTGAAGTCATATGATTGGCTAGAGCCTCAACTGACCG
AACCAGAGGATCCATCACAATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCANAAATGTAGA
   
Sequence Name 12-K111556w-0022-740-F11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K111556w-0022-740-F11C-8409
TTGATTGTGATGGATCCTCTGGTTCGGTCAGTTGAGGCTCTAGCCAATCATATGACTTCA
CATGCATTGAACCATAAACGAGCTTGCTTAGCACGGTCATGCCCGGATGGTTATGAAGAG
GTATCATAGAAGAAGGTGGCATACAGAAGATTCCTATCTGTTTTGACAACAGAGGCAAAA
TGAACCAAGTGAGAGATAACATTGGTGGCAGTATCAGGAAGATAAAACACCCAACTAGAG
ACAGACTCAGAATTGACTCGAACTGTGTTTACTCATACTTTCTAGACCATTTGTCATAGG
CTTTTTTGCAGACTTCAGGCTCATGGCTTGAATAGTTGGTTCCTGAGATTCTCTCAAGGA
CCATGTTTGGATGCCCCCCTATTCCCCTAATTTTGAATATGCGGAAAAGGAGAAGATTAA
ATATCCACTAAAATTTTTGTCTCAGGATTGGCTATAGAGTGTTTTAACCTTGAACGGTAT
TTGCATATTATTCCAAGCAGAACCATTGCTACTTAAACCTAGGAAGTTAAAATGGGACTC
TTATGCTATTTTGTATCTGAATCATCCTAAAGTGGATCACAAAAGATTCACCCAAACCTT
AAACAGCATTCAGACCCAATCA

Last Updated on 06.12.2018 16:11