Sequences from confirmation amplicon


Confirmation sequences for line 741B04 and hit At1g80400 are shown.
Line ID 741B04
Locus At1g80400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30226098
Primers used for PCR 31uv - 8474, show primer details
   
Sequence Name 89-K117671-0022-741-B04C-31uv
GenBank Accession LN514981 [GenBank]
Sequencing primer gene specific primer, 31uv: 5'-CGAGAGCTAGAGTTGAATTTGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K117671-0022-741-B04C-31uv
TTGTGATTTTCGTTTTCACTTGGATTAGATGGATGTTTCTTCCATTGTGCGAAATACAAC
CCTAACTGATCAAGATCCTTTGTTAATGGACCACGGTAACCGCGAGCACATCGTTGACAT
TACAAGCAATGACGAGGAGGACTCTTCATCAAGATCTTCTCTCGATGAGCTTACTCCTCA
AGGTAACTCACATCAAGGTGAAGAGACAGTATTATCATCATCAAATGCTACACAACAACA
CCCAACTCATCCATCTCCTCCTCTCCTGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCG
   
Sequence Name 41-K117670-0022-741-B04C-8409
GenBank Accession LN504238 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117670-0022-741-B04C-8409
GCATTTACAATTGAATATATCCGGAGAGGAGGAGATGGATGAGTTGGGTGTTGTTGTGTA
GCATTTGATGATGATAATACTGTCTCTTCACCTTGATGTGAGTTACCTTGAGGAGTAAGC
TCATCGAGAGAAGATCTTGATGAAGAGTCCTCCTCGTCATTGCTTGTAATGTCAACGATG
TGCTCGCGGTTACCGTGGTCCATTAACAAAGGATCTTGATCAGTTAGGGTTGTATTTCGC
ACAATGGAAGAAACATCCATCTAAATCCAAGTAGAAAACTGAAAATCACAAAAGTCAGCA
ACCACAAGTCCCCATTGTGTTGACCAAAGCAAATTCAACTCTAGCTCTCG

Last Updated on 06.12.2018 16:11