Sequences from confirmation amplicon


Confirmation sequences for line 741C04 and hit At1g77920 are shown.
Line ID 741C04
Locus At1g77920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29299331
Primers used for PCR AC40 - 8474, show primer details
   
Sequence Name 23-K027917-022-741-C04C-8409
GenBank Accession LN496423 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K027917-022-741-C04C-8409
CATTTACAATTGAATATATCCTGCCATAAACAAATGCATAAACAATAGATCAAAACAAAG
CTAATTATGTTTTCTTTTTTATACATGCATAATAGAAAATGGTGGTTTTTGTGATATTGA
TTGATATTCTTTTGTCATATGCCAATAGATGAAACGGCGTTTAGCGCAGAACCGAGAAGC
GGCTCGCAAAAGTCGTTTGAGAAAGAAGGTAGAAGCAAGTCCCTTTATTGAATCTCATAT
AGTTTAGTTAGTTGCTACTAAGTCCTCTTTTTTTTTGTATCTTTAGGCTTATGTTCAGCA
GTTAGAGGAAAGCCGGTTAAAGTTATCGCAGTTAGAGCAAGAACTCGAAAAGGTTAAGCA
GCAGGTGAGCTAGGTCAAAAGATATGTTTAAAATGTTCTGTTTTGAGTCTCTATCAAGAA
AATCAGTTTTGGTTTCGTAATTGACTCAGTACTAAATGTTTTCTTTCAAGGGCCATTTAG
GACCATCT
   
Sequence Name 24-K027917-022-741-C04C-AC40
GenBank Accession LN496861 [GenBank]
Sequencing primer gene specific primer, AC40: 5'-CCTGTGTTAATACTCCCAGATGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K027917-022-741-C04C-AC40
CTGAGTCAATTCGAAACCAAAACTGATTTTCTTGATAGAGACTCAAAACAGAACATTTTA
AACATATCTTTTGACCTAGCTCACCTGCTGCTTAACCTTTTCGAGTTCTTGCTCTAACTG
CGATAACTTTAACCGGCTTTCCTCTAACTGCTGAACATAAGCCTAAAGATACAAAAAAAA
AGAGGACTTAGTAGCAACTAACTAAACTATATGAGATTCAATAAAGGGACTTGCTTCTAC
CTTCTTTCTCAAACGACTTTTGCGAGCCGCTTCTCGGTTCTGCGCTAAACGCCGTTTCAT
CTATTGGCATATGACAAAAGAATATCAATCAATATCACAAAAACCACCATTTTCTATTAT
GCATGTATAAAAAAGAAAACATAATTAGCTTTGTTTTGATCTATTGTTTATGCATTTGTT
TATGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11