Sequences from confirmation amplicon


Confirmation sequences for line 741D05 and hit At4g34290 are shown.
Line ID 741D05
Locus At4g34290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16411484
Primers used for PCR 07gj - 8474, show primer details
   
Sequence Name 33-K111630-0022-741-D05C-07gj
GenBank Accession LN501047 [GenBank]
Sequencing primer gene specific primer, 07gj: 5'-TCGCAGAGTATATCCCTCTTGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K111630-0022-741-D05C-07gj
CCCCAAATTCCACACAGATAGATAAAAGATATACATTACTATTGTCATAAAAAATGTTTC
TTTTGTTGAAATTTTAGCACAATGAACACTTAAGAATCTATACAGAACAAGTAAGAGTAG
ATTACAACATGAGAAAAAAGTACAAACTAACGTCTTAAGGTCAAACCCCAGTATGGACAT
GTCTACATTTGTTTCCATTACACAGCACAAAGAGTCATTACACAGGGACTAGTATAATGC
CAAATGCATAGGTAATACATTGGTCAAGTACAATGATCAAAGAACATCAAAAGTCATTAC
CAAAACATATCCACTAAAGTTTATCATCACAACAATGATAAAATAATTTGAAGCAATTAA
CCAGTCATATCAAAAGAAGTTCAAAACCCCAAATTCAATCTCACAATCCGTTGAATCCTT
CAAAAGTTTATTATAACAAACATGAAAGAAACAAATACGGTAAGTAACATGACGACTAAT
CACAAGAACCTGTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCA
   
Sequence Name 17-K111629-0022-741-D05C-8409
GenBank Accession LN493908 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K111629-0022-741-D05C-8409
TTTACAATTGAACAGGTTCTTGTGATTAGTCGTCATGTTACTTACCGTATTTGTTTCTTT
CATGTTTGTTATAATAAACTTTTGAAGGATTCAACGGATTGTGAGATTGAATTTGGGGTT
TTGAACTTCTTTTGATATGACTGGTTAATTGCTTCAAATTATTTTATCATTGTTGTGATG
ATAAACTTTAGTGGATATGTTTTGGTAATGACTTTTGATGTTCTTTGATCATTGTACTTG
ACCAATGTATTACCTATGCATTTGGCATTATACTAGTCCCTGTGTAATGACTCTTTGTGC
TGTGTAATGGAAACAAATGTAGACATGTCCATACTGGGGTTTGACCTTAAGACGTTAGTT
TGTACTTTTTTCTCATGTTGTAATCTACTCTTACTTGTTCTGTATAGATTCTTAAGTGTT
CATTGTGCTAAAATTTCAACAAAAGAAACATTTTTTATGACAATAGTAATGTATATCTTT
TATCTATCTGTGTGGAATTTGTGGGCTAACTGAATAATGTTTCTTCTCCCAGGACCCACA
AAACAAGAGGGATTACCTCTGCGA

Last Updated on 06.12.2018 16:11