Sequences from confirmation amplicon


Confirmation sequences for line 741G02 and hit At4g12460 are shown.
Line ID 741G02
Locus At4g12460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7395757
Primers used for PCR 59sm - 8474, show primer details
   
Sequence Name 42-K116518-0022-741-G02C-59sm
GenBank Accession LN504601 [GenBank]
Sequencing primer gene specific primer, 59sm: 5'-AAGGAGCAATCGATTCTTGATAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K116518-0022-741-G02C-59sm
GCACTGTTAGTGTTTTCGATCTACGTGGCATTTCTGAAATAACTTCATGGTCAAATATAT
ATAGGTTAATGGTTTTGTAGAAGATGTAACTGGAAAAAAGGCTGCGACGGTATTTGGTAA
ATGGAATGATAGCCTTTATTATGTTGCTGGTGATGGAATCAACAAGGCAAGCGCCTCATT
ACTGTGGAAAGCGACAAAGGCACCACCTAATGTCACCAGATACAACTTTACATCATTCGC
GATGACGCTAAATGAATTGATACCTGGTTTGGAGGTATATTAGTATTTTTTTAATTTTGG
ATTTGTTTGTAAACATGAATTTTGGGTTTCTGCTGTACTTGAAATCTTGTTTTAGCAACT
GTGTGATCTTTTCGTAATTTTGTTCCCTTGGTTTAAGGAAAAGCTGCCTCCAACAGATTC
TAGGCTCCGACCAGATCAACGACACTTGGAGAACGGTGAATATGAGAAGGCAAATGAAGA
AAGAAAGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
ATCCGCA
   
Sequence Name 16-K116517-0022-741-G02C-8409
GenBank Accession LN493587 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116517-0022-741-G02C-8409
TCTTTCTTTCTTCATTTGCCTTCTCATATTCACCGTTCTCCAGTGTCGTTGATCTGGTCG
GAGCCTAGAATCTGTTGGAGGCAGCTTTTCCTTAAACCAAGGGAACAAAATTACGAAAAG
ATCACACAGTTGCTAAAACAAGATTTCAAGTACAGCAGAAACCCAAAATTCATGTTTACA
AACAAATCCAAAATTAAAAAAATACTAATATACCTCCAAACCAGGTATCAATTCATTTAG
CGTCATCGCGAATGATGTAAAGTTGTATCTGGTGACATTAGGTGGTGCCTTTGTCGCTTT
CCACAGTAATGAGGCGCTTGCCTTGTTGATTCCATCACCAGCAACATAATAAAGGCTATC
ATTCCATTTACCAAATACCGTCGCAGCCTTTTTTCCAGTTACATCTTCTACAAAACCATT
AACCTATATATATTTGACCATGAAGTTATTTCAGAAATGCCACGTAGATCGAAAACACTT
AACAGTGCATATCTCTGTGTAATCACCTGGTGGGGATTTCTATCAAGAATCGATTGCTCC
T

Last Updated on 06.12.2018 16:11