Sequences from confirmation amplicon


Confirmation sequences for line 741G07 and hit At4g02410 are shown.
Line ID 741G07
Locus At4g02410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1060789
Primers used for PCR NL57 - 8474, show primer details
   
Sequence Name 12-K103979-0022-741-G07C-NL57
GenBank Accession LN491548 [GenBank]
Sequencing primer gene specific primer, NL57: 5'-TCTGTCAGAACATTTTGTTTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K103979-0022-741-G07C-NL57
GGAGAAGCTCCCCGTTGACTTTTCGAAAGTTCCGAAGTTTCCGAGGCTCGGGCCAACGAG
TTTACAGAGGTTTTATAAGAACAGGATGCCGTTATTTTCCCTCTTGTTGATTCCGGTCTT
GTTTGTTGTCTCGCTCATCTTCCTCGTGCGCTTTATCGTGAGGAGGAGGAGAAAGTTCGC
GGAGGAGTTCGAAGATTGGGAAACAGAGTTTGGGAAGAACCGGTTAAGGTTTAAGGACTT
GTACTATGCTACAAAAGGGTTCAAGGATAAGGACCTTCTTGGATCGGGTGGGTTTGGTAG
GGTTTATAGAGGTGTCATGCCGACAACGAAGAAAGAGATCGCGGTGAAGAGAGTGTCGAA
TGAATCTCGACAAGGGTTGAAAGAGTTTGTTGCTGAGATTGTGAGTATTGGTAGGATGAG
TCATCGGAACTTAGTTCCTCTCTTGGGTTATTGTCGTCGTAGAGACGAGCTTCTTTTGGT
GTATGATTACATGCCTAATGGTAGCTTAGACAAGTATTTATAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAAGAAAGAGTAATTACCAATTTTTTTTCATTTCAAAAATGTAGATGTCCGC
   
Sequence Name 28-K103980-0022-741-G07C-8409
GenBank Accession LN498814 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K103980-0022-741-G07C-8409
TTCCGGAATGAGCCATTTACATTGAATATATCCTATAAATACTTGTCTAAGCTACCATTA
GGCATGTAATCATACACCAAAAGAAGCTCGTCTCTACGACGACAATAACCCAAGAGAGGA
ACTAAGTTCCGATGACTCATCCTACCAATACTCACAATCTCAGCAACAAACTCTTTCAAC
CCTTGTCGAGATTCATTCGACACTCTCTTCACCGCGATCTCTTTCTTCGTTGTCGGCATG
ACACCTCTATAAACCCTACCAAACCCACCCGATCCAAGAAGGTCCTTATCCTTGAACCCT
TTTGTAGCATAGTACAAGTCCTTAAACCTTAACCGGTTCTTCCCAAACTCTGTTTCCCAA
TCTTCGAACTCCTCCGCGAACTTTCTCCTCCTCCTCACGATAAAGCGCACGAGGAAGATG
AGCGAGACAACAAACAAGACCGGAATCAACAAGAGGGAAAATAACGGCATCCTGTTCTTA
TAAAACCTCTGTAAACTCGTTGGCCCGACCCTCGGAAACTTCGGAACTTTCGATAAAGTC
AACGGCGGAGCTGTCTTCCCTTTCACCATGAAACTCCACCCAAAAACAAAA

Last Updated on 06.12.2018 16:11