Sequences from confirmation amplicon


Confirmation sequences for line 742F07 and hit At4g17210 are shown.
Line ID 742F07
Locus At4g17210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9653929
Primers used for PCR BH56 - 8474, show primer details
   
Sequence Name 33-K104383-0022-742-F07C-BH56
GenBank Accession LN500983 [GenBank]
Sequencing primer gene specific primer, BH56: 5'-AGTTCAGCTAGTCTCTCTTCCACG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K104383-0022-742-F07C-BH56
TATTCCGCGAGCTCGTATTTCGCCATGAACAGCTCCGCGGTAATCAAAATGTAATCCTCT
CTTGTGCTCTCTGTTTCTTGGTTTTCAGATTGAAGTTGTTCTAACCGTTGATTCATGGTG
TGTTTGGTAGAGATAGCACATTTCCTTGAATGTTTGATGGATTCTAGTTTAACCCTTAAA
TCAGCAGCCTTTCGTTGAGCAGAGTCGAGATCAGAGAGAGCTTGTGCTTTAGCTGCGACA
GAGCCATCAAGACATATCTTGACTCTATCCATTTCTTGTAATGTAATTAAAATTTTCATT
TCAGTTGCACATACACTTTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 41-K104384-0022-742-F07C-8409
GenBank Accession LN504068 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K104384-0022-742-F07C-8409
CCATTTACATTGAATAATCCTGAAAAAGTGTATGTGCAACTGAAATGAAAATTTTAATTA
CATTACAAGAAATGGATAGAGTCAAGATATGTCTTGATGGCTCTGTCGCAGCTAAAGCAC
AAGCTCTCTCTGATCTCGACTCTGCTCAACGAAAGGCTGCTGATTTAAGGGTTAAACTAG
AATCCATCAAACATTCAAGGAAATGTGCTATCTCTACCAAACACACCATGAATCAACGGT
TAGAACAACTTCAATCTGAAAACCAAGAAACAGAGAGCACAAGAGAGGATTACATTTTGA
TTACCGCGGAGCTGTTCATGGCGAAATACGAGCTCGCGGAATTAAAACAACAGTTTAACC
TCTCCGTGGAAGAGAGATA

Last Updated on 06.12.2018 16:11