Sequences from confirmation amplicon


Confirmation sequences for line 742F12 and hit At5g08350 are shown.
Line ID 742F12
Locus At5g08350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2686637
Primers used for PCR GC55 - 8474, show primer details
   
Sequence Name 21-K033540-022-742-F12C-8409
GenBank Accession LN495568 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K033540-022-742-F12C-8409
TTACATTGAATATATCCTGGTATCCACCTTGAGGGGAATCCACCTTTATTGATCTCTCGC
TGCAGAACGCCATTTTCTTGGAAGAGATGAAGAGAAGGCCGGCTATGGGACCCGCGGTTG
TCGACAAGTAACACTGGTACATTTTGAACAGTTTCTCACCTTCACTAACTCTAAAGAGCC
GCTTGAAGATCTTCTCTAAACCTCCAACCTGAAGAATCCTAGCGCCCAAAGATAGCTTTC
TCTTCACAGTTTCGGTTAGCTTTGGTCTTATCTTGCTCTGGTCTCTAACTCCGTTGGTGA
ACCCATCAGTTTTCTTCCTTTTAAGAATCGATTTTACGTTGCCTGTCAAAAAAAAAAAAT
CAATCCTGTTAGCTTTCAAGTTTTCCAGGGACAAAACAATATGAATTAGAGGGTAACTAA
ATAGGTACTAACTTTGTTCAAATTTCTTGGAAAAAGCCGGAACTCGGAACTTGTTAAATG
AAGCCGGATCAGGTAAGTAACCCACGGGAGTAGCCTTTGCGGCCGGATATCTAATGACTT
GTTGTTCAACTCTGCTCAGGTTCATCTTTAGAGGATTGAGTAATTGATCTAGATCTTGAA
TCTTGAGGTTGTG
   
Sequence Name 21-K033540-022-742-F12C-GC55
GenBank Accession LN495569 [GenBank]
Sequencing primer gene specific primer, GC55: 5'-TTTCCTTATCACACAACCTCAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K033540-022-742-F12C-GC55
CATCCTCTAAGATGACATGAGCAGAGTTGAACACAAGTCATTAGATATCCGGCCGCAAAG
GCTACTCCCGTGGGTTACTTACCTGATCCGGCTTCATTTAACAAGTTCCGAGTTCCGGCT
TCTTCCAAGAAATCTGAACAAAGTTAGTACCTATTTAGTTACCCTCTAATTCATATTGTT
TTGTCCCTGGAAAACTTGAAAGCTAACAGGATTGATTTTTTTTTTTTGACAGGCAACGTA
AAATCGATTCTTAAAAGGAAAAAAACTGATGGGTTCACCAACGGAGTTAGAGACCAAAGC
AAGATAAGACCAAAGCTAACCGAAACTGTGAAAAGAAAGCTATCTTTGGGCGCTAGGATT
CTTCAGGTTGGAGGTTTAAAGAAAATCTTCAAGCGGCTCTTTAAAGTTAGTGAAGGTGAA
AAACTGTTCAAAATGTACCAGTGTTACTTGTCGACAACCGCGGGTCCCATAGCCGGCCTT
CTCTTCATCTCTTCCAAGAAAATGGCGTTCTGCAGCGAGAGATCAATAAAGGTGGATTCC
CCTCAAGGTGGATACCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAAATGTCCGCACCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11