Sequences from confirmation amplicon


Confirmation sequences for line 743G01 and hit At5g09360 are shown.
Line ID 743G01
Locus At5g09360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2906637
Primers used for PCR BC60 - 8474, show primer details
   
Sequence Name 23-K104153-0022-743-G01C-BC60
GenBank Accession LN496531 [GenBank]
Sequencing primer gene specific primer, BC60: 5'-GTCGACATTCTCCGAGCTTATTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K104153-0022-743-G01C-BC60
TTTCCCGAGAATCCGCCGACGAAATTCACTACACCGGTGAGAATCTTCCATTTCCGACTA
GGTTCGGGACGAAAGTTGTGGTTCTCGATTATAACTCGAGCGTTGAGCTGATTCTACAGG
GGACGAACGTGTTGGCTTCAGATAATCACCCGATTCATCTGCATGGGTATAACTTCTACG
TCGTGGGTTCGGGTTTTGGGAATTTCGATCGTCGTAAAGATCCGTTGAGGTATAACCTAG
TTGATCCACCGGAGGAGACAACCGTCGGTGTTCCTCGTAACGGTTGGACCGCCGTCAGAT
TCGTAGCTNATAATCCAGGTCAGTTCACACTCTACGTTTCTCAAACGTTTCACGTGAAAA
TATATATCAATTTTGCATTAGTTTATACAAAAGATATTATTGTTTAAATGAAAAATATGA
ATTTATTAGATGAGTAAATATGGCTTCAAACAAAAGAAAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 23-K104154-0022-743-G01C-8409
GenBank Accession LN496532 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K104154-0022-743-G01C-8409
GCCATTTTTCTTTTGTTTGAGCCATATTTACTCATCTAATAAATTCATATTTTTCATTTA
AACAATAATATCTTTTGTATAAACTAATGCAAAATTGATATATATTTTCACGTGAAACGT
TTGAGAAACGTAGAGTGTGAACTGACCTGGATTATTAGCTACGAATCTGACGGCGGTCCA
ACCGTTACGAGGAACACCGACGGTTGTCTCCTCCGGTGGATCAACTAGGTTATACCTCAA
CGGATCTTTACGACGATCGAAATTCCCAAAACCCGAACCCACGACGTAGAAGTTATACCC
ATGCAGATGAATCGGGTGATTATCTGAAGCCAACACGTTCGTCCCCTGTAGAATCAGCTC
AACGCTCGAGTTATAATCGAGAACCACAACTTTCGTCCCGAACCTAGTCGGAAATGGAAG
ATTCTCACCGGTGTAGTTGAATTTCGTCGGCGGATTTCTCGGGAAATCCTCCTGGAAAAC
GCCTCCAATGTGGCGATNATAAGCTCGGAGAAAGTCGA

Last Updated on 06.12.2018 16:11