Sequences from confirmation amplicon


Confirmation sequences for line 743H12 and hit At5g17200 are shown.
Line ID 743H12
Locus At5g17200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5654599
Primers used for PCR CE87 - 8474, show primer details
   
Sequence Name 13-K106018-0022-743-H12C-CE87
GenBank Accession LN492044 [GenBank]
Sequencing primer gene specific primer, CE87: 5'-CAAGTCTTCGACCATCTCCTTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K106018-0022-743-H12C-CE87
TGTTCAATTAATTGGAAATCCAGCCCTAATGTGAATGATTTCAATAGTGATCGATTTTAG
CTTATGAGTAGACAATAACTCGAACCAATGTTTAAATAATTTGGTGATTTATTTACATTA
GATAAGAGGTAATCTTGTGGCACCGGGTTACACTTGGTATGCGGGAAGATACACAACATG
GATTAGTTTTGACAGTATCAATGGTCTTGTTGTCACCGGAGGTGGTACCATCGACGGTCG
TGGTTCTTTGTGGTGGGGCAACGTTAATAATCGCCCCTGCGTAAGTCCATTTGCAAGATC
CTCACATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCACGATTATTA
   
Sequence Name 13-K106019-0022-743-H12C-8409
GenBank Accession LN492045 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K106019-0022-743-H12C-8409
GCCATTTACATTGAATATGTGAGGATCTTGCAAATGGACTTACGCAGGCGCTATTATTAA
CGTTGCCCCACCACAAAGAACCACGACCGTCGATGGTACCACCTCCGGTGACAACAAGAC
CATTGATACTGTCAAAACTAATCCATGTTGTGTATCTTCCCGCATACCAAGTGTAACCCG
GTGCCACAAGATTACCTCTTATCTAATGTAAATAAATCACCAAATTATTTAAACATTGGT
TCGAGTTATTGTCTACTCATAAGCTAAAATCGATCACTATTGAAATCATTCACATTAGGG
CTGGATTTTCCAATATAATTTGAAACAAGAGAAAGAGACAAGTATATATATACCGATAAG
GAGATGGTCGAAGACTT
Primers used for PCR CE88 - 8474, show primer details
   
Sequence Name 85-K118414-0022-743-H12C-CE88
GenBank Accession LN514589 [GenBank]
Sequencing primer gene specific primer, CE88: 5'-CGAAATCTCCAGTTACTCCAAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K118414-0022-743-H12C-CE88
AATGATTAGCATGTACCTTGCTGTATATTATATGATGATTGTTTGTGAATCTGTGATTTT
AGGAATCACCTGATACCGTGACCGGGGCCACAAAAAATGCCGGTTATGTTGATATGAGAG
GAACCGCTATTAATGGCGATGCAGTCATCTCCAGTGGCAATCACTGAGTCATGTATGTGG
ACTCCTATACAGTTCGATATGTCAATACCATCCGTGTTTGGACTATCACCGGGTGCTGTC
ATCCTTAAACCCCTGACCTCGATGTTCTGTGAACAACTTAGACCTACATGGTTCCGAGGG
CTGTTCAAATGTCGGAGATTAGATATCCTAAGACCATTACAGTTGTTGAAGTGCATTGCC
TAAGAAAAGCAAGGAAGAAAAAGAAACATCATTAATAACTGAGAATAAATATTTGATCGA
AGTTCATATTCAATTGCAATTGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATATCCG
   
Sequence Name 37-K118413-0022-743-H12C-8409
GenBank Accession LN502784 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K118413-0022-743-H12C-8409
CATTGCATTGAATATGAACTTCGATCAAATATTTATTCTCAGTTATTAATGATGTTTCTT
TTTCTTCCTTGCTTTTCTTAGGCAATGCACTTCAACAACTGTAATGGTCTTAGGATATCT
AATCTCCGACATTTGAACAGCCCTCGGAACCATGTAGGTCTAAGTTGTTCACAGAACATC
GAGGTCAGGGGTTTAAGGATGACAGCACCCGGTGATAGTCCAAACACGGATGGTATTGAC
ATATCGAACTGTATAGGAGTCCACATACATGACTCAGTGATTGCCACTGGAGATGACTGC
ATCGCCATTAATAGCGGTTCCTCTCATATCAACATAACCGGCATTTTTTGTGGCCCCGGT
CACGGTATCAGGTGATTCCTAAAATCACAGATTCACAAACAATCATCATATAATATACAG
CAAGGTACATGCTAAATCATTTATAGTATGGATTTGATATTATAGCGTAGGAAGTCTTGG
AGTAACTGGNAGATTTCG

Last Updated on 06.12.2018 16:11