Sequences from confirmation amplicon


Confirmation sequences for line 744A09 and hit At1g65220 are shown.
Line ID 744A09
Locus At1g65220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24228354
Primers used for PCR 59lm - 8474, show primer details
   
Sequence Name 36-K114022-0022-744-A09C-59lm
GenBank Accession LN502306 [GenBank]
Sequencing primer gene specific primer, 59lm: 5'-CGCTTAATACTGATATGATTGCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K114022-0022-744-A09C-59lm
CTTTTGTCACTAGTTTGTTGTGTTCAACAATTCTCAGTTACTTCTGATTCTGTATTCGAT
TATCACTTGTCTTTTATCTCTGCTTTCTTATTGTATATGTAAAATTGATATATCTGCTAT
ACTTTTCCTTCTGATTTTAAGTAAGGCAGGATTGACAGCTCTGGTAGAGTACAATGAAAG
GAAAATATTTGAGGTGAAGCTGAAGGAAATCAAAGCGGTCCTTACGAGCCAAGTGACAGA
GGAAATAAACGTAGATGAAGTGACTGAAATGGTGAAGCAACAGGTGAAAGATGCAAAGCT
GCCAGAGACTGAGGTTGTGCATGTGATCTGGGATGGGATAATGAATGCTGTTCAATGGTC
TGGGAAAAACCAGCAGCAGAATTCGAATGCTGTATTACGCCAAGTGAGTTGATTTACAAT
TGAATGCTGTATTACGCCATGTGTTCATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 15-K114021-0022-744-A09C-8409
GenBank Accession LN493060 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K114021-0022-744-A09C-8409
ATTTCATTGATATGAACACATGGCGTAATACAGCATTCAATTGTAAATCAACTCACTTGG
CGTAATACAGCATTCGAATTCTGCTGCTGGTTTTTCCCAGACCATTGAACAGCATTCATT
ATCCCATCCCAGATCACATGCACAACCTCAGTCTCTGGCAGCTTTGCATCTTTCACCTGT
TGCTTCACCATTTCAGTCACTTCATCTACGTTTATTTCCTCTGTCACTTGGCTCGTAAGG
ACCGCTTTGATTTCCTTCAGCTTCACCTCAAATATTTTCCTTTCATTGTACTCTACCAGA
GCTGTCAATCCTGCCTTACTTAAAATCAGAAGGAAAAGTATAGCAGATATATCAATTTTA
CATATACAATAAGAAAGCAGAGATAAAAGACAAGTGATAATCGAATACAGAATCAGAAGT
AACTGAGAATTGTTGAACACAACAAACTAGTGACAAAAGTTAACCTATATAGAAGCATTT
TCACTAATTTAATCTTATCAATGAGAAATGGCAATCATATCA

Last Updated on 06.12.2018 16:11