Sequences from confirmation amplicon


Confirmation sequences for line 744F08 and hit At3g01480 are shown.
Line ID 744F08
Locus At3g01480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:188345
Primers used for PCR CD08 - 8474, show primer details
   
Sequence Name 25-K029489-022-744-F08C-8409
GenBank Accession LN497310 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K029489-022-744-F08C-8409
AGCCATTTACAATTGAATATATCCTGATTTCATTGCTTCTTTCTCTCTACACTCTGGATA
AGATTTCAATTTTCTTCCGGATTCTTTCTTTTTGTAATTTATTATGATGGGCCGTTAGTT
GGGCTTTCTTTGCACGATCTGTTTAGTTATCATCTTTTTCTGTGATTTACTATGATGGGT
CGTTAGTTGGGCTTTGTTTGTATTATCTGTTAGTTATAGAATCATAGTGAATCCGTTATG
TGATTTCGGTTTCGACTTTTCTGAAAGAAAAAAAACTAACACAACACATATATGTAATTT
CTTTGTGATGAAAATGTTAATTTCATTTTCTTCATTTGTTTGGAAGTGGACTTCATTAGA
GTCTTGCATTATCACCTTACGTCCAATATTAGTGACATACTGACATCTTTAAATGAAGTC
AACACAAACGTAATTTACATATAAATATAGTGGGGGGAGGGGGCTAATATTGTAGCTGGT
ACTTTATTCATGCTATAAATAATCCAGTCTTTAATAAGGTATTTTTTTGATTAGTTGATT
ACTTAAAGATGTATGCT
   
Sequence Name 26-K029489-022-744-F08C-CD08
GenBank Accession LN497787 [GenBank]
Sequencing primer gene specific primer, CD08: 5'-CATGAGCAATTGAGCATACATCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K029489-022-744-F08C-CD08
TACCTTATTAAGACTGGATTATTTATAGCATGAATAAAGTACCAGCTACAATATTAGCCC
CCTCCCCCCACTATATTTATATGTAAATTACGTTTGTGTTGACTTCATTTAAAGATGTCA
GTATGTCACTAATATTGGACGTAAGGTGATAATGCAAGACTCTAATGAAGTCCACTTCCA
AACAAATGAAGAAAATGAAATTAACATTTTCATCACAAAGAAATTACATATATGTGTTGT
GTTAGTTTTTTTTCTTTCAGAAAAGTCGAAACCGAAATCACATAACGGATTCACTATGAT
TCTATAACTAACAGATAATACAAACAAAGCCCAACTAACGACCCATCATAGTAAATCACA
GAAAAAGATGATAACTAAACAGATCGTGCAAAGAAAGCCCAACTAACGGCCCATCATAAT
AAATTACAAAAAGAAAGAATCCGGAAGAAAATTGAAATCTTATCCAGAGTGTAGAGAGAA
AGAAGCAATTGAAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11