Sequences from confirmation amplicon


Confirmation sequences for line 744G12 and hit At2g28305 are shown.
Line ID 744G12
Locus At2g28305 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12083499
Primers used for PCR 14ay - 8474, show primer details
   
Sequence Name 58-K107248-0022-744-G12C-14ay
GenBank Accession LN509720 [GenBank]
Sequencing primer gene specific primer, 14ay: 5'-TAGCAGAAGGAGCAGATACAATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K107248-0022-744-G12C-14ay
ATGAACCTTCTTCCCTGCTTTGTCTTGAATGATAACAATGAATTGTAGTATCCTTCCACA
TTCAACAGTCCCACCTTCACATTCATAACATTAAGAATTATTATTGGTAAAAAAAAAACT
AATAACATGGCATTTAAGAAACTAGTAATGACTTAAATTTTCATTTGACGAATTTATCAA
TCATTTACGATTTTCGTCACGATCGATTAATCGTTCGTTATAGATTATTTCTAATGTGAA
CGTCTAATTTACTAAAAGAAAATTAGAGTTTTAGAGTTTACTTTGGTTAGGACGTATGTG
AAAAAAAGATGTCCATAAAATAGAACTAAAATAACGTGTTACCTAAAAAATAATGTATTC
TCTTTAGGAGATTTTCTTTCACTATTTGATGGCAATAGTTATTTTTCCTTGTTAATTACC
TAGTTTAGTCTAAGATTACTTCAAGTTATTAGGAATGTATAATGTAATATATATAAATAG
AAATGGAAGCAAAATGATTACTGGTTTATCATGGATGCCTAGCTGTGCCCAAGTGATAAC
TTCAAGAAGCTCTTCAAGTGTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 42-K107247-0022-744-G12C-8409
GenBank Accession LN504481 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K107247-0022-744-G12C-8409
GCCATTTACATTGAATATATCCTGGAACACTTGAAGAGCTTCTTGAAGTTATCACTTGGG
CACAGCTAGGCATCCATGATAAACCAGTAATCATTTTGCTTCCATTTCTATTTATATATA
TTACATTATACATTCCTAATAACTTGAAGTAATCTTAGACTAAACTAGGTAATTAACAAG
GAAAAATAACTATTGCCATCAAATAGTGAAAGAAAATCTCCTAAAGAGAATACATTATTT
TTTAGGTAACACGTTATTTTAGTTCTATTTTATGGACATCTTTTTTTCACATACGTCCTA
ACCAAAGTAAACTCTAAAACTCTAATTTTCTTTTAGTAAATTAGACGTTCACATTAGAAA
TAATCTATAACGAACGATTAATCGATCGTGACGAAAATCGTAAATGATTGATAAATTCGT
CAAATGAAAATTTAAGTCATTACTAGTTTCTTAAATGCCATGTTATTAGTTTTTTTTTTA
CCAATAATAATTCTTAATGTTATGAATGTGAAGGTGGGACTGTTGAATGTGGAAGGATAC
TACAATTCATTGTTATCATTCATAGACAAAGCAGTGGAAGAAGGTTTCATTTCACCAACT
GCTCGTCATATCATTGTATCTGCTCCTTCTGCT

Last Updated on 06.12.2018 16:11