Sequences from confirmation amplicon


Confirmation sequences for line 744H05 and hit At1g03740 are shown.
Line ID 744H05
Locus At1g03740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:935825
Primers used for PCR 30ve - 8474, show primer details
   
Sequence Name 30-K117967-0022-744-H05C-30ve
GenBank Accession LN499903 [GenBank]
Sequencing primer gene specific primer, 30ve: 5'-GTTAGTTACCTCCACGGTCATTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117967-0022-744-H05C-30ve
TTGACAGAGAGTTATTTGCTTTGACCGGTGGTACAAATTTTCTCTCGTGTGATATTCTCG
TCATAGAGTCTTGTCTTTCTTGTTTCTCCGCCCTCATAGGTTGTTGCCTTTTCGCTTCAT
CACGCATTTTCGCATCAATCTCTTTACTAGGAGGATATTTTGGTAAGTTCGATGGATCGC
AAGCAAACGGCTTGGTTTTGAAGTACTGACAAAAGAACAGAGTTTTGTCACTAATGCTTC
CATGATCTCAGTAAACCAAGAACATAGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 14-K117966-0022-744-H05C-8409
GenBank Accession LN492702 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117966-0022-744-H05C-8409
TTCTTGGTTACTGAGATCATGGAAGCATTAGTGACAAAACTCTGTTCTTTTGTCAGTACT
TCAAAACCAAGCCGTTTGCTTGCGATCCATCGAACTTACCAAAATATCCTCCTAGTAAAG
AGATTGATGCGAAAATGCGTGATGAAGCGAAAAGGCAACAACCTATGAGGGCGGAGAAAC
AAGAAAGACAAGACTCTATGACGAGAATATCACACGAGAGAAAATTTGTACCACCGGTCA
AAGCAAATAACTCTCTGTCAATGACAATGGAGGTAAGTAACTGTAAAAGGTCCTAGCTTT
TATGGTCACAATGACCGTGGAGGTTAACTAAC

Last Updated on 06.12.2018 16:11