Sequences from confirmation amplicon


Confirmation sequences for line 745C07 and hit At5g47380 are shown.
Line ID 745C07
Locus At5g47380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19223115
Primers used for PCR KA25 - 8474, show primer details
   
Sequence Name 08-K106147-0022-745-C07C-KA25
GenBank Accession LN489739 [GenBank]
Sequencing primer gene specific primer, KA25: 5'-TGAGCAGGTACACCATATGCTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K106147-0022-745-C07C-KA25
TAGCAGATGTAAAGGGATTTTGTATCAGATCAATATATTCTTACATGCATGAGGAGAGCA
TTGTATATGTTGATCCAAAAGGCTAACTTTGCATTGCCTTCCATTTGATTTATAGTAACT
CTCTCTAGTTGTTCAACAAGGAGCCTGCAAGTTAAAATCATGAGTTCTTGTGATGGAACT
AAATTCAGATGTTGTTTTCAAAAACTGCTATTGTTGGACTAACCTATAGTTGTTGATAGC
ATACGTTACTTGAGAAAATCTTTTCTTGTCTGATGAGATCCAAGATACTTCCACCATGGA
TCTGCAGGACCAAGCTCTGTCTTCATTCATAATGTTCTTGGGGATGATCACATTGCTAGT
AGATGATCTTGATAGGATTCTCTTTTCAGGATCTGCTGACATGGCACTGCAGCAGAGCCA
GAAGTACACTGAAGACATACACTTGACCATATCTTCAGACAACTTGTTTGGGCATTGGTA
CAAATGATCCTTAAGAGTTCTCTGAGATGGAGACTTCACTGTTACTGAATCCTTTAAATA
ACAATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 24-K106148-0022-745-C07C-8409
GenBank Accession LN497001 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K106148-0022-745-C07C-8409
TTTAAGGATTCAGTAACAGTGAAGTCTCCATCTCAGAGAACTCTTAAGGATCATTTGTAC
CAATGCCCAAACAAGTTGTCTGAAGATATGGTCAAGTGTATGTCTTCAGTGTACTTCTGG
CTCTGCTGCAGTGCCATGTCAGCAGATCCTGAAAAGAGAATCCTATCAAGATCATCTACT
AGCAATGTGATCATCCCCAAGAACATTATGAATGAAGACAGAGCTTGGTCCTGCAGATCC
ATGGTGGAAGTATCTTGGATCTCATCAGACAAGAAAAGATTTTCTCAAGTAACGTATGCT
ATCAACAACTATAGGTTAGTCCAACAATAGCAGTTTTTGAAAACAACATCTGAATTTAGT
TCCATCACAAGAACTCATGATTTTAACTTGCAGGCTCCTTGTTGAACAACTAGAGAGAGT
TACTATAAATCAAATGGAAGGCAATGCAAAGTTAGCCTTTTGGATCAACATATACAATGC
TCTCCTCATGCATGTAAGAATATATTGATCTGATACAAAATCCCTTTACATCTGCTAATT
CATGCTTCTATGAGGATAATCTTCTATATATCTTCTCAAGGCATACTTAGCATATGGTGT
ACCTGCTC
Primers used for PCR 30au - 8474, show primer details
   
Sequence Name 67-K118446-0022-745-C07C-30au
GenBank Accession LN511905 [GenBank]
Sequencing primer gene specific primer, 30au: 5'-ACTTATCACGGAGATTGAGTTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K118446-0022-745-C07C-30au
ATCATGTGCTTTCTNATATCGAAGTATATTTGAACAGACAGTAAGCAGAGCACCTTCCGA
ACAAAGCTCAAGCATTTCTTCTCCAGCTCATCATATAAAGCAGCCACCAAGAAAACAAGA
CCCAAATGTTATTTCAAATGCATTTTGTTCCTCCAATAATTTCCCTCTAAAGCCGTGGCA
TGCTATGGTTACTCTTAAAGATTCGAGTAGAAAAACTTCCAAAAAGGACCAATCTTCTCA
GTTCCAGTTCAGAAACTGTATTCCTTCAACAACAAGTTGCTCAAGTCAAGCAAAAGTAAA
TGAATAAAGACTAACTTCCCCCTAATTTAGTCATTGTTGATACTTCTTCTAACTTTTATA
TATTCTGTTGATTTTTCTGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAAGGTTTA
   
Sequence Name 27-K118445-0022-745-C07C-8409
GenBank Accession LN498601 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118445-0022-745-C07C-8409
CATTTACAATTGACAGAAAAATCAACAGAATATATAAAAGTTAGAAGAAGTATCAACAAT
GACTAAATTAGGGGGAAGTTAGTCTTTATTCATTTACTTTTGCTTGACTTGAGCAACTTG
TTGTTGAAGGAATACAGTTTCTGAACTGGAACTGAGAAGATTGGTCCTTTTTGGAAGTTT
TTCTACTCGAATCTTTAAGAGTAACCATAGCATGCCACGGCTTTAGAGGGAAATTATTGG
AGGAACAAAATGCATTTGAAATAACATTTGGGTCTTGTTTTCTTGGTGGCTGCTTTATAT
GATGAGCTGGAGAAGAAATGCTTGAGCTTTGTTCGGAAGGTGCTCTGCTTACTGTCTGTT
CAAATATACTTCGATATAGAGAAAGCACATGATGCTCGCGATTCGTGACCTCTGCTTCAA
GCAACTCAATCTCCATGATAAG

Last Updated on 06.12.2018 16:11