Sequences from confirmation amplicon


Confirmation sequences for line 746B08 and hit At1g07795 are shown.
Line ID 746B08
Locus At1g07795 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2414462
Primers used for PCR CR78 - 8474, show primer details
   
Sequence Name 40-K107114-0022-746-B08C-8409
GenBank Accession LN503725 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K107114-0022-746-B08C-8409
TTGAAGCCTTAGCGATGGAAGACGGTGATACATCTTGATCAGAAGAAATGGGGATTGTTT
TAGCGTGGATCAAAAGAAATTAGGTGTTGGTTTGCGAGGAAACGAAAAGATTGTGAGATC
GATGAGCAAAAACCAGAGCTTGTTTGATTGGTGCGGTTTATGTGTTTGGAAACGGAAATG
AAAATGGAAAGGCTGCTTCTTTGCACGTTTAACTAACTACTCTTCTTTCCCATACACACA
CAGAGAGTTGTTAATTACGAATTTGCCCTTCCCTTTGAACTCTTCTCTTCTTCTTTTGCC
CTTCCGGGTCGTCTCCGTCCCCTTTGACACTCCCACGTTTTCAGCAATATCAATTTTTGT
TTAATCGACGGCTGTATTAGTTGGTTGTTAAGAAATTTCAGACTCGTTCCTCCATAATTC
AGATATAGGAGGAGAACATAAACTATATTATATGATTATGGAGTAATGACAGTGATTTAT
GCTTTATGTTGTTCACCAAAAACTATTCATTAAAATTAAGCGGGTTCACCTAAATCTGAT
GCTTATAGCCTGCTTGTGGCACAACGATNACATTAGGTA
   
Sequence Name 56-K107115-0022-746-B08C-CR78
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, CR78: 5'-ATTACCTATCGTTATCGTTGTGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K107115-0022-746-B08C-CR78
AAAAAAAAGAAAGAGTAATTACCTTTTTTTTTTCAATTCAAAAATGT

Last Updated on 06.12.2018 16:11