Sequences from confirmation amplicon


Confirmation sequences for line 747A10 and hit At1g49750 are shown.
Line ID 747A10
Locus At1g49750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18412142
Primers used for PCR 28tq - 8474, show primer details
   
Sequence Name 52-K116920-0022-747-A10C-28tq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 28tq: 5'-AGGTACTTGATGTTGCCTATGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K116920-0022-747-A10C-28tq
CCATTTCGAGATAAAGATCCAGCTCTTACCGATTTTTTCCCCATTCAAAAATGTAGATTC
CGCCCGCCCTCAACAAAAA
   
Sequence Name 08-K116919-0022-747-A10C-8409
GenBank Accession LN489899 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K116919-0022-747-A10C-8409
ATTTACATTGATCCATCAAGCTCTGTGCCTGATTTCAGCAATTTGAAATTCTTATACGAG
CTCGATCTAAGCAACAACAAACTCACAGGAGATTTCCCAACTAGTGTCTTGAAAGGAAAC
AATCTCACGTTTCTTGATCTCAGGTTCAATTCTTTCTCAGGCTCTGTTCCTCCTCAGGTC
TTTAATCTCGACCTCGACGTCTTGTTCATCAACAACAACAATCTTGTTCAGAAGCTTCCA
CTCAATCTTGGATCCATCACTGCTCTTTACCTCACCTTCGCCAACAACAGGTTAGTTTCT
CAACTAGTTGATCTAGTCAGAGGTGCAAGAATAGAGAGAGTGTGGTTGAATGTGTGATTA
TTGAGTAGCTAGATTATTATTTTTAATATTTGTTGTGATGTGACTTAGGTTCACGGGTCC
AATTCCCGAAAGCATAGGCAA

Last Updated on 06.12.2018 16:11