Sequences from confirmation amplicon


Confirmation sequences for line 747H02 and hit At5g66460 are shown.
Line ID 747H02
Locus At5g66460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26540850
Primers used for PCR AP19 - 8474, show primer details
   
Sequence Name 53-K106187-0022-747-H02C-AP19
GenBank Accession LN508161 [GenBank]
Sequencing primer gene specific primer, AP19: 5'-CAGCCGTATCATCAGATAAACAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K106187-0022-747-H02C-AP19
GAAATAATTCCATAAACAAGGAGATACCCAAATTATTGGTCTGATCCAACTGGTGCATTG
ATAAGTGTATATTCAATTTCTGATTAAACCATTTTTCCAAAGTTCTTCCAACTGAATCAA
AACATAACATAACACACACAGACAAGTTGGGTTTAATGGAGGTTACCTGAAACATATCCT
CGTTGTAGGAGCCAGGGGAATACTGAAGAGCCCTGTAACCGCCATCGCTGAAAGCCCAGG
TTCGAGCAACGGTCAATCCATGGCGAGAAGCATCTTGGAAAGCGGTGGAGATCTTAGACC
GTTGGGATGGATCGGAGGCTACGTACATGAGCCAGTAGGCATTGAAGCCATTAGCGTAAT
ATGGATAGCCATTGAGACTAAACTGAACACCTTTCGTCCTCACGAACCCACCGCTCGGTA
ATGCATCTGTTCCTAGCTCCCAACAGCTGAGTTGGATCACGATCGCTAGAAATGGAAACA
GAGCCAGAAGCTTCATCTTTTTCTTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 37-K106188-0022-747-H02C-8409
GenBank Accession LN502622 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K106188-0022-747-H02C-8409
ATTTACAATTGAAGAAAAAAAGATGAAGCTTCTGGCTCTGTTTCCATTTCTAGCGATCGT
GATCCAACTCAGCTGTTGGGAGCTAGGAACAGATGCATTACCGAGCGGTGGGTTCGTGAG
GACGAAAGGTGTTCAGTTTAGTCTCAATGGCTATCCATATTACGCTAATGGCTTCAATGC
CTACTGGCTCATGTACGTAGCCTCCGATCCATCCCAACGGTCTAAGATCTCCACCGCTTT
CCAAGATGCTTCTCGCCATGGATTGACCGTTGCTCGAACCTGGGCTTTCAGCGATGGCGG
TTACAGGGCTCTTCAGTATTCCCCTGGCTCCTACAACGAGGATATGTTTCAGGTAACCTC
CATTAAACCCAACTTGTCTGTGTGTGTTATGTTATGTTTTGATTCAGTTGGAAGAACTTT
GGAAAAATGGTTTAATCAGAAATTGAATATACACTTATCAATGCACCAGTTGGATCAGAC
CAATAATTTGGGTATCTCCTTGTTTATGGAATTAATTTCTGTGAATCTTTTTGGACAAAT
TTGAGATTCTCTGTTTATCT

Last Updated on 06.12.2018 16:11