Sequences from confirmation amplicon


Confirmation sequences for line 747H12 and hit At5g37810 are shown.
Line ID 747H12
Locus At5g37810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15045411
Primers used for PCR KN34 - 8474, show primer details
   
Sequence Name 63-K103224-0022-747-H12C-KN34
GenBank Accession LN510963 [GenBank]
Sequencing primer gene specific primer, KN34: 5'-CCTACAAATGTGCAACTCCAACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K103224-0022-747-H12C-KN34
ATCTCCCATAACATATGATCCTACTTTTCATCTGTTGCATCCAATCACAAGTCTACTATT
CCAAATCATTAGATTCAATCCAATACTTCTGTGATTGTTTCATTTAAGGTATATTTCATT
TTAAAAAAAAACTCTTTATCACTAAAGTTTTCGTTTATTTTTTCTTTCTGAAATAAAAAT
CTCTCCATTCAGCTTTAGGAAAAAAAAAATTTGTTTCTAAAAACAAATCCAAAAAATGTC
TTCGCATAGTGATGAAATCGAAAAAAAACAAATATCAGGAATTGAGAAAGGCAAAGGCAA
AGATTGCCAAGGAGGAATCGAGACGGTTATATGTACTTCTCCCAGCATTGTCTGCCTTAC
CCAAAAGGTTTTAAAATAAAATTTTATTGTAATTATGGTGTTCGGGAAATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATTTCCGCAC
   
Sequence Name 31-K103225-0022-747-H12C-8409
GenBank Accession LN500121 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K103225-0022-747-H12C-8409
TTCGGACTGAGCCATTTACATTGAATATTTCCCGAAACCATAATTACAATAAAATTTTAT
TTTAAAACCTTTTGGGTAAGGCAGACAATGCTGGGAGAAGTACATATAACCGTCTCGATT
CCTCCTTGGCAATCTTTGCCTTTGCCTTTCTCAATTCTTGATATTTGTTCTTCTTCGATT
TCATCACTATGCGAAGACATTTTTTGGATTTGTTCTTAGAAACAAATTTTTTTTTTCCTA
AAGCTGAAGGGAGAGATTTTTATTTCAAAAAGAAAAAATAAACGAAAACTTTAGTGATAA
AGAGTTTTTTTTTAAAATGAAATATACCTTAAATGAAACAATCACAGAATTATTGGATTG
AATCTAAGGATTTGGAATAGTAAACTTGTGATTGGATGCAACAAATAAAAAGTAGGATCA
TATGTTATGTGGAGATGGGTAAAAAATGCTAAAATGGGAGTCTAGTTGGAGTTGCAATTT
TGTAGG
Primers used for PCR KN35 - 8474, show primer details
   
Sequence Name 08-K103729-0022-747-H12C-KN35
GenBank Accession LN489699 [GenBank]
Sequencing primer gene specific primer, KN35: 5'-TATCAATTTGGTGCGATCATTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K103729-0022-747-H12C-KN35
CGCCCACGCTATCAGCCTCTATCTACAATTTGCTAATTACCCTTGCAATATTTTTAGCAA
ATGACGAGCTAATCTACATGATATTTATTTTTTGGAATAGCTATGAAAATGACTGCTTTA
ATATTGCATAAGATGAGAAAATTGTTCATTTTGTATTTTTTGAAAAAGGAATCTTCTTCT
TGACGTTGAACTTGTAATGTTTGAAACAATCTAGATCTTATTCGATCAAGGCTAAAAGAA
TAGATGATGACCTGATGCCAAGGAAACCGTCGGAAAATGGCGAAAGTAACGGTAACGGCA
GGGTTAAAATGTGCACCGGAAATGTGACCAGTAGAGTAAATCATGACCATAACAATGAGA
CCCCAAGTAACACAGATCCCTGGAAATGTTATTGTTCCTCCGTACAAGACATTCACTACC
ACCACTCCACATCCNGAGAATACTATAAAATAGGTCCCAATCATCTCTGCTATCAACTGT
AATTAATTTTAAAGGAAAACACCATAAATCAATTTGCGCAAAATTTTGTTATCATTACAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 24-K103730-0022-747-H12C-8409
GenBank Accession LN496958 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K103730-0022-747-H12C-8409
TCGGACTGAGCCTGTAATGATACAAAATTTTGCGCAAATTGATTTATGGTGTTTTCCTTT
AAAATTAATTACAGTTGATAGCAGAGATGATTGGGACCTATTTTATAGTATTCTCTGGAT
GTGGAGTGGTGGTAGTGAATGTCTTGTACGGAGGAACAATAACATTTCCAGGGATCTGTG
TTACTTGGGGTCTCATTGTTATGGTCATGATTTACTCTACTGGTCACATTTCCGGTGCAC
ATTTTAACCCTGCCGTTACCGTTACTTTCGCCATTTTCCGACGGTTTCCTTGGCATCAGG
TCATCATCTATTCTTTTAGCCTTGATCGAATAAGATCTAGATTGTTTCAAACATTACAAG
TTCAACGTCAAGAAGAAGATTCCTTTTTCAAAAAATACAAAATGAACAATTTTCTCATCT
TATGCAATATTAAAGCAGTCATTTTCATAGCTATTCCAAAAAATAAATATCATGTAGATT
AGCTCGTCATTTGCTAAAAATATTGCAAGGGTAATTAGCAAATTGTAGATATGAGGCTGA
TTAGCGTGGGGCTGTGAACATTGTACTTTAATTTAATGATCGCACCAAATTGAT

Last Updated on 06.12.2018 16:11