Sequences from confirmation amplicon


Confirmation sequences for line 749B08 and hit At2g36870 are shown.
Line ID 749B08
Locus At2g36870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15473697
Primers used for PCR 17fb - 8474, show primer details
   
Sequence Name 88-K111271-0022-749-B08C-17fb
GenBank Accession LN514878 [GenBank]
Sequencing primer gene specific primer, 17fb: 5'-CATAGAATTTTTGGGGACAACATT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K111271-0022-749-B08C-17fb
GAGGAAGTGGTGATGGGAAAATCATTGGCCGTGAGATGAAGTTTCGCTTGTGGTTTGATC
CAACTAAAGATTTTCACCATTATGCTATTCTTTGGAGCCCTAGAGAAATCATGTAAGCCT
TCTTCTACTTAATGTCTCCTACAAAAATGGTTTTTATATTTTTCATTTTGTTGAATCTAA
CCCTTTAATAACGATAGTGTATATAAGATCCGTGTTCTTACGAGAAGATACGACAAATTC
ATTTTTTGGCCACGGACCTAGGTGGATGTATCTAGAGCCATATAGTAAACAATAAAAATA
GACATACGTTACATAACTTTTATTTTATTTCCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCACGNTTATT
   
Sequence Name 73-K111270-0022-749-B08C-8409
GenBank Accession LN512921 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K111270-0022-749-B08C-8409
GCATTTCATTGAATATATCCTGGAAATAAAATAAAAGTTATGTAACGTATGTCTATTTTT
ATTGTTTACTATATGGCTCTAGATACATCCACCTAGGTCCGTGGCCAAAAAATGAATTTG
TCGTATCTTCTCGTAAGAACACGGATCTTATATACACTATCGTTATTAAAGGGTTAGATT
CAACAAAATGAAAAATATAAAAACCATTTTTGTAGGAGACATTAAGTAGAAGAAGGCTTA
CATGATTTCTCTAGGGCTCCAAAGAATAGCATAATGGTGAAAATCTTTAGTTGGATCAAA
CCACAAGCGAAACTTCATCTCACGGCCAATGATTTTCCCATCACCACTTCCTCTAATATA
CACATTTGTCTGAAGTGTGTAAGGCTTCCCAAATGTTGTCCCCAAAAATTCTATG

Last Updated on 06.12.2018 16:11