Sequences from confirmation amplicon


Confirmation sequences for line 749C02 and hit At3g27970 are shown.
Line ID 749C02
Locus At3g27970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10390696
Primers used for PCR NL63 - 8474, show primer details
   
Sequence Name 66-K103789-0022-749-C02C-NL63
GenBank Accession LN511593 [GenBank]
Sequencing primer gene specific primer, NL63: 5'-GTAGAATCCATCAAGAGAGATGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K103789-0022-749-C02C-NL63
TATCCTCTTTTTATATTTAATAATTAAACTTTTAACCTATATGAATCGAACGCAAGAATA
TATACTTATTCAAATCATATGATTTTCTCATTTTCAGGGATTGACGACTCGAATGGCAGC
TTTAGGCTTAAGAGATAAGGCCATGATCGACTACACGTCATCACGGTCTCCAAGAGTGGT
TGCACTCTCTTGCAAGATGGTAGGAGGAGGAAGTGACGGGTCGTTGGATCTATGCGCGAG
GGTTTGCATAACGGATGAGAGTGACAACGTTATCTTTCACACGTATGTGAAACCTTCGAT
GGCCGTGACTAGCTATAGGTACGAGACGACNGGGATACGCCCGGAAAATCTAAGGGACGC
AATGCCNTTAAAACAAGTACAAAGAAAGATTCAAGAGTTTCTTTGTAATGGAGAACCCAT
GTGGAAGATTCGTCCAAGAGGTGGAAAAGCGAGGATTCTCGTGGGGCATGGCCTCGATCA
CATGTAAATGAGTATGAAATCTACATGTAAATGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCANAAATATAGATATCCGCA
   
Sequence Name 74-K103790-0022-749-C02C-8409
GenBank Accession LN513062 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K103790-0022-749-C02C-8409
CATTTACATTGAATATATCCATTTACATGTAGATTTCATACTCATTTACATGTGATCGAG
GCCATGCCCCACGAGAATCCTCGCTTTTCCACCTCTTGGACGAATCTTCCACATGGGTTC
TCCATTACAAAGAAACTCTTGAATCTTTCTTTGTACTTGTTTTAATGGCATTGCGTCCCT
TAGATTTTCCGGGCGTATCCCTGTCGTCTCGTACCTATNGCTAGTCACGGCCATCGAAGG
TTTCACATACGTGTGAAAGATAACGTTGTCACTCTCATCCGTTATGCAAACCCTCGCGCA
TAGATCCAACGACCCGTCACTTCCTCCTCCTACCATCTTGCAAGAGAGTGCAACCANCTC
TNGGAGAACCGTGATGAACGTGTAGTCGATCATGNGCCTTATCTNCTNNAAGCCTAANGC
TGCCATTTCGAGTCGTCAATCCNCTGAAAATGAAGAAAATTCATATGAATTTGAATAAGT
TATATATTTCTTGGCGTTTCGATTCANNATNGGTTTAAAAGTTTTAATTTTATATAAATA
TGAAAAAAGAAGGATTAACAGAAGTTGACGNCCTCGAGAATTNNGGCATCTCTCTAAGAT
GGAATTCTA

Last Updated on 06.12.2018 16:11