Sequences from confirmation amplicon


Confirmation sequences for line 749C12 and hit At1g63990 are shown.
Line ID 749C12
Locus At1g63990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23743571
Primers used for PCR IF23 - 8474, show primer details
   
Sequence Name 11-K032413-022-749-C12C-8409
GenBank Accession LN491000 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K032413-022-749-C12C-8409
GAGCCTTTACAATTGAAATGGATCAGCATGTATAGTTGGTTACCTGCAATATTTTGGAGG
ACAATAAGCTCTTAGCAATCTGTGAATCTTTTGGCTTTAAGGGAACCAAAGACTCTTCTG
GTATCAGATTCAGATCATCTCCTCGGAGACCAATCCACTTCACATTGCAAGCTAAAGAGG
ATGCAGAAATTATCATTGTAGAGACTTGGAGATTATTTGGTGCAAACAGCTAAGTCTTTA
CCGTATCTGTATGCTTCAAGTCCCATCCCTATGCTTCCAAACTTGAAGGTGCATAGTATA
GCTAACCCAGCTGGATTCCTGACCTCTCATTTATGAACATATGAGCACTAAAGTTTTATG
TTAACAAAGAGGAAGATGTTTATTTATGCTTACCAATCAACTAGAACAAGAATTGGCAGA
TCAGGAAAAGTTGTGCTCATCCGGTGGAGGAAAAACCTAAAAGATTTAACTTCCAAGCTA
CTTAATACCACTGACTACATTGCAATATGGTTTTGTAACTGAGTTGAAGAATTGACCTTG
TGGCAATATCCGGATACCCTTTCGCTGTGATGAACACGCAAGGAATGTGATTGAACACAC
GATCTTCCACGAGCCGATGAAAGATCGCATGCTAAAAACAGATTCTAGGAGTGAGTTACA
GACAATTACGACATCTCTCATATGTGCTTGATTACATCAAGAAGCT
   
Sequence Name 11-K032413-022-749-C12C-IF23
GenBank Accession LN491001 [GenBank]
Sequencing primer gene specific primer, IF23: 5'-GTGGAAAAGGTAGCTTCTTGATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K032413-022-749-C12C-IF23
TATTGTCTGTACTCACTCCTAGAATCTGTTTTTAGCATGCGATCTTTCATCGGCTCGTGG
AAGATCGTGTGTTCAATCACATTCCTTGCGTGTTCATCACAGCGAAAGGGTATCCGGATA
TTGCCACAAGGTCAATTCTTCAACTCAGTTACAAAACCATATTGCAATGTAGTCAGTGGT
ATTAAGTAGCTTGGAAGTTAAATCTTTTAGGTTTTTCCTCCACCGGATGAGCACAACTTT
TCCTGATCTGCCAATTCTTGTTCTAGTTGATTGGTAAGCATAAATAAACATCTTCCTCTT
TGTTAACATAAAACTTTAGTGCTCATATGTTCATAAATGAGAGGTCAGGAATCCAGCTGG
GTTAGCTATACTATGCACCTTCAAGTTTGGAAGCATAGGGATGGGACTTGAAGCATACAG
ATACGGTAAAGACTTAGCTGTTTGCACCAAATAATCTCCAAGTCTCTACAATGATAATTT
CTGCATCCTCTTTAGCTTGCAATGTGAAGTGGATTGGTCTCCGAGGAGATGATCTGAATC
TGATACCAGAAGAGTCTTTGGTTCCCTTAAAGCCAAAAGATTCACAGATTGCTAAGAGCT
TATTGTCCTCCAAAATATTGCAGGTAACCAACTATACATGCTGATCCATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG

Last Updated on 06.12.2018 16:11