Sequences from confirmation amplicon


Confirmation sequences for line 750A10 and hit At5g59550 are shown.
Line ID 750A10
Locus At5g59550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23999110
Primers used for PCR BG40 - 8474, show primer details
   
Sequence Name 18-K101744-0022-750-A10C-8409
GenBank Accession LN494243 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K101744-0022-750-A10C-8409
CGGAATGAGCCTTTACAATTGCGCTAATTGGAGGCTAATTGCGCTGTTTGCACCGAGATT
TTCGAGACGGAGACGGAGGCGCGTGAGATGCCGTGTAAACACCTTTTCCACGACGATTGC
ATCGTACCGTGGCTATCGATTCGAAACTCTTGTCCCGTGTGCAGATTCGAGCTACCTTCA
GAGCCTAATCGACGGAGCAACAACAACGAGGAGGATAACGCCGTGGGGATGACGATTTGG
AGACTTCCCGGTGGTGGATTCGCCGTAGGGAGGTTTAACGCAGCAATGAGAGACGGAGAG
AGAGTTTTGCCAGTGGTGTTAACAGAAATGGATGGTGGTGGGATTGGTAATAGCCAGGAT
GGTCCTAGACGGATCTCATGGGTTAGGTCACACGGTACACTCGAATCGGATAGTAACGGT
ACTGGCTCAGGTTCCGGCTCCGGTGGAAGATTGAGGAGGATGGTTCGTGGAATGGTGTCG
TTAATGAGGAGAGTGAGACCAAACCGTAGCTCTTCTTCTTCATCAGCTGCGATATCAACT
TCTTCTGATAATCTGGATTTGAACATTGAAGTAGAATCTAGGGTTTTGGATAGGTCAAAT
TCAGTGATGAGAAGATACTTCAGAAGAAACAGAAGTAACAGGGATTCTTCGTCTTCAGTT
CTTCATTGATTCAGAAATATATATATATTATTTTTTGGTTTGTTCATTCAACAATGTAAA
TATAAGATTCAGAAGAAAAAGATTGGTGCTCGGTTTTTC
   
Sequence Name 18-K101750-0022-750-A10C-BG40
GenBank Accession LN494244 [GenBank]
Sequencing primer gene specific primer, BG40: 5'-TAGAAAACGAAGCACCAATCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K101750-0022-750-A10C-BG40
TTGTTGATGAACAACCAAAAAATAATATATATATATTTCTGAATCAATGAAGAACTGAAG
ACGAAGAATCCCTGTTACTTCTGTTTCTTCTGAAGTATCTTCTCATCACTGAATTTGACC
TATCCAAAACCCTAGATTCTACTTCAATGTTCAAATCCAGATTATCAGAAGAAGTTGATA
TCGCAGCTGATGAAGAAGAAGAGCTACGGTTTGGTCTCACTCTCCTCATTAACGACACCA
TTCCACGAACCATCCTCCTCAATCTTCCACCGGAGCCGGAACCTGAGCCAGTACCGTTAC
TATCCGATTCGAGTGTACCGTGTGACCTAACCCATGAGATCCGTCTAGGACCATCCTGGC
TATTACCAATCCCACCACCATCCATTTCTGTTAACACCACTGGCAAAACTCTCTCTCCGT
CTCTCATTGCTGCGTTAAACCTCCCTACGGCGAATCCACCACCGGGAAGTCTCCAAATCG
TCATCCCCACGGCGTTATCCTCCTCGTTGTTGTTGCTCCGTCGATNAGGCTCTGAAGGTA
GCTCGAATCTGCACACGGGACAAGAGTTTCGAATCGATNGCCACGGTACGATGCANTCGT
CGTGGAAAAGGTGTTTACACGGCATCTCANGCGCCTCCNTCTCCGTCTCGAANATCTCCG
TGCAAACAGCGCANTTAGCCTCCAATTAGCGCANTTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCANAAATGTAGATTCCG
Primers used for PCR BG41 - 8474, show primer details
   
Sequence Name 19-K101744-0022-750-A10C-OriL
GenBank Accession LN494700 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K101744-0022-750-A10C-OriL
CGGAATGAGCATTTACAAGCCGATTTAGACGCCGGAGGGTTTCCAGATCTACCACTTCCA
GTAGCCGACGCTTCGATTTGGCTTAGCTGTTCAAGTAACCGCTCGAATCCAGATCCCATC
AAGATCTCAGATACAGAATCCGGAAGCGGTCTAAGACCCGATCCGGATCCGTCATCGTAA
TAAAACTCAAAAGCGCGTCGATCTCTAGCCGCATCTCCTTCTTCTCCCTCTTCTCTCTCG
CCGGCGCCTCCTTGAAGTACGATGACCGGATTAAAGGACGGACGACGACCGGATCTCCGG
CGTCTAATCACGGATCTACGGTTGTTATTGATCGATCTTACTTCAGTAGAAGCCGGAATC
GTTAATTCGGTAGCGGCGGAGGAAGAATCGTTAATCTGTTCGATAAAACCGCCGTCGCAG
TGAGGGCAAGCGACGCTACCGACGGTTGTTGTGCCTTGATCAGCCCAAACGCTGACGAAA
CGCGTACAGCTGTAACACCAATACGAAGCCGTGATCGGCGTTGTTGTAGTCGGCGTCGTC
GGCGTTGACGGCATCTTTAAAATCTTCTTACTCGGTGAAAGGATCTGAGAAGAAGAAGAA
AGAAGAATCGCTGACCAGAGTAATGACNGAGAGGTTTGAAGATGAGA
   
Sequence Name 19-K101750-0022-750-A10C-BG41
GenBank Accession LN494701 [GenBank]
Sequencing primer gene specific primer, BG41: 5'-CACGTTTTCATTTTCTCATCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K101750-0022-750-A10C-BG41
CGATTCTTCTTTCTTCTTCTTNCAGATCCTTTCACCGAGTAAGAAGATTTTAAAGATGCC
GTCAACGCCGACGACGCCGACTACAACAACGCCGATCACGGCTTCGTATTGGTGTTACAG
CTGTACGCGTTTCGTCAGCGTTTGGGCTGATCAAGGCACAACAACCGTCGGTAGCGTCGC
TTGCCCTCACTGCGACGGCGGTTTTATCGAACAGATTAACGATTCTTCCTCCGCCGCTAC
CGAATTAACGATTCCGGCTTCTACTGAAGTAAGATCGATCAATAACAACCGTAGATCCGT
GATTAGACGCCGGAGATCCGGTCGTCGTCCGTCCTTTAATCCGGTCATCGTACTTCAAGG
AGGCGCCGGCGAGAGAGAAGAGGGAGAAGAAGGAGATGCGGCTAGAGATCGACGCGCTTT
TGAGTTTTATTACGATGACGGATCCGGATCGGGTCTTAGACCGCTTCCGGATTCTGTATC
TGAGATCTTGATGGGATCTGGATTCGAGCGGTTACTTGAACAGCTAAGCCAAATCGAAGC
GTCGGCTACTGGAATTGGTAGATCTGGAAACCCTCCGGCGTCTAAATCGGCTTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAN
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGT

Last Updated on 06.12.2018 16:11