Sequences from confirmation amplicon


Confirmation sequences for line 750D03 and hit At1g23110 are shown.
Line ID 750D03
Locus At1g23110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8196994
Primers used for PCR CR80 - 8474, show primer details
   
Sequence Name 84-K107250-0022-750-D03C-CR80
GenBank Accession LN514437 [GenBank]
Sequencing primer gene specific primer, CR80: 5'-ATGTCACGTCTCATTCTCCTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K107250-0022-750-D03C-CR80
TTCGACCAACACCAAATCCTCTGTCATGGAATCAAATCAAAGACTTTCCATCGAGTATGT
TACATAACATTAGGTTGTGACATTCTCAGCTTTTGAAACGGTTTCAGTTACTGATTTAGT
TTCTCTGTTACAGACGTTTTTGGCAGCAAAGACCTCCATGCTTAAGGCCAATCTGTTGCA
GCATTCGCGGTACGCTTCTTGTTTTCGATCTTTTATCTTTCCAATCTCAAGATGGTTGAT
CGGTCTTTTTATTATGCAATCGAAACATATGACAGGTGACCAGAGTGTCTTAGAAACAGC
TGCTAATGTGGCTACTTCACTTCCTTTCATCTTCCTCGGAATGCAGGCACCAAGGTAAAA
TGTTCTCTTTGCAAATATCATTTCAAGTAGAGTTTCTCTGACAAGATACTATCCCATGTA
GGAAGAATCTGAACACAAAGGTGTACGCAAACTCCCTAATTGGAGTTGGAATTGCGTCGA
GTCTGTATCATGCCTCTAGGGGAAAGTTGAGGAAGTACCTGAGATGGGTAGATTACACAA
TGATAGCCACAACAACAATGTATTCAAAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 68-K107249-0022-750-D03C-8409
GenBank Accession LN512028 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K107249-0022-750-D03C-8409
CATTTTTGATACATTGTTGTTGTGGCTATCATTGTGTAATCTACCCATCTCAGGTACTTC
CTCAACTTTCCCCTAGAGGCATGATACAGACTCGACGCAATTCCAACTCCAATTAGGGAG
TTTGCGTACACCTTTGTGTTCAGATTCTTCCTACATGGGATAGTATCTTGTCAGAGAAAC
TCTACTTGAAATGATATTTGCAAAGAGAACATTTTACCTTGGTGCCTGCATTCCGAGGAA
GATGAAAGGAAGTGAAGTAGCCACATTAGCAGCTGTTTCTAAGACACTCTGGTCACCTGT
CATATGTTTCGATTGCATAATAAAAAGACCGATCAACCATCTTGAGATTGGAAAGATAAA
AGATCGAAAACAAGAAGCGTACCGCGAATGCTGCAACAGATTGGCCTTAAGCATGGAGGT
CTTTGCTGCCAAAAACGTCTGTAACAGAGAAACTAAATCAGTAACTGAAACCGTTTCAAA
AGCTGAGAATGTCACAACCTAATGTTATGTAACATACTCGATGGAAAGTCTTTGATTTGA
TTCCATGACAGAGGATTTGGTGTTGGTCTGAAAGTCACTAACTTGAGTTGTCTTCTCAAA
TGAGAAAGGAGAATGAGACGTGACA

Last Updated on 06.12.2018 16:11