Sequences from confirmation amplicon


Confirmation sequences for line 750E02 and hit At2g25930 are shown.
Line ID 750E02
Locus At2g25930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11060573
Primers used for PCR BA23 - 8474, show primer details
   
Sequence Name 72-K104767-0022-750-E02C-BA23
GenBank Accession LN512722 [GenBank]
Sequencing primer gene specific primer, BA23: 5'-TTCGATGAAAGTTTCTTGTGATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K104767-0022-750-E02C-BA23
TCCTTTTTACCCTCTTCAATGCAGCCTTGTGGTGTGGAAAGAAACTTATCTGTCCAGCAT
CTTGATTCTTCAGCCGCAAACCAAGCAACTGAGAAGTTTGTCTCCCAAATGTCCTTCATG
GAAAATGTGAGATCTTCGGCACAGCATGATCAGAGGAAAATGGTGAGAGAGGAAGAAGAT
TTTGCAGTTCCAGTATATATTAACTCAAGAAGATCTCAGTCTCATGGCAGAACCAAGAGT
GGTATTGAGAAGGAAAAACACACCCCAATGGTGGCACCTAGCTCTCATCACTCCATTCGA
TTTCAAGAAGTGAATCAGACAGGCTCAAAGCAAAACGTATGTTTGGCTACTTGTTCAAAA
CCTGAAGTTAGGGATCAGGTCAAGGCGAATGCAAGGTCAGGTGGCTTTGTAATCTCTTTA
GATGTATCAGTCACAGAGGAGATTGATCTCGAAAAATCAGCATCAAGTCATGATAGAGTA
AATGACTTCATGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 72-K104768-0022-750-E02C-8409
GenBank Accession LN512723 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K104768-0022-750-E02C-8409
GCCTTTACAATTGAATATATCCTGACATGAAGTCATTTACTCTATCATGACTTGATGCTG
ATTTTTCGAGATCAATCTCCTCTGTGACTGATACATCTAAAGAGATTACAAAGCCACCTG
ACCTTGCATTCGCCTTGACCTGATCCCTAACTTCAGGTTTTGAACAAGTAGCCAAACATA
CGTTTTGCTTTGAGCCTGTCTGATTCACTTCTTGAAATCGAATGGAGTGATGAGAGCTAG
GTGCCACCATTGGGGTGTGTTTTTCCTTCTCAATACCACTCTTGGTTCTGCCATGAGACT
GAGATCTTCTTGAGTTAATATATACTGGAACTGCAAAATCTTCTTCCTCTCTCACCATTT
TCCTCTGATCATGCTGTGCCGAAGATCTCACATTTTCCATGAAGGACATTTGGGAGACAA
ACTTCTCAGTTGCTTGGTTTGCGGCTGAAGAATCAAGATGCTGGACAGATAAGTTTCTTT
CCACACCACAAGGCTGCATTGAAGAGGGTAAAAAAGGATTATTTCTCATGTCCTTGCAAT
AGTCTGAATGCTCAGGGCAATCACAAGAAACTTTCATCGA
Primers used for PCR BA22 - 8474, show primer details
   
Sequence Name 73-K104767-0022-750-E02C-BA22
GenBank Accession LN512895 [GenBank]
Sequencing primer gene specific primer, BA22: 5'-CACAGTCCTCAATGAAAATAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K104767-0022-750-E02C-BA22
TAGACTACGGAACATGCTTAATATTAACTTCTTTCAGCAAAAAATGTTGGTATCAAAGAA
GGAGATAAATAGAAAGAGCGGAGAATAAATAACCAAACTGTAAATTTCTAGTGAACTTAC
TTGGCAATGGCTTTCCTTGCTCTCCAGAAACGTTTTTGACCTAATATACCCACAACATCA
TCGGGAGAGACATCTATGCTGGATATAGAATCCACCATCGAATCATCAGAAACGTCATCA
CTTGCCTCTTCATTTATCTGCTGCAGAGAGGCGCATGCTCTGCTTTTGCTGTATTCACGA
TCATTATCAATGTCTTCAGGACTGCCATGACCCTCTTGTGAATGATTTTCCGTTGCCAAG
TGAGATTCAGCTCCATTATCAGTGACTTCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCAGCAACGTTATTA
   
Sequence Name 73-K104768-0022-750-E02C-OriL
GenBank Accession LN512896 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K104768-0022-750-E02C-OriL
TTTACATTGAATAATCCTGAAGTCACTGATAATGGAGCTGAATCTCACTTGGCAACGGAA
AATCATTCACAAGAGGGTCATGGCAGTCCTGAAGACATTGATAATGATCGTGAATACAGC
AAAAGCAGAGCATGCGCCTCTCTGCAGCAGATAAATGAAGAGGCAAGTGATGACGTTTCT
GATGATTCGATGGTGGATTCTATATCCAGCATAGATGTCTCTCCCGATGATGTTGTGGGT
ATATTAGGTCAAAAACGTTTCTGGAGAGCAAGGAAAGCCATTGCCAAGTAAGTTCACTAG
AAATTTACAGTTTGGTTATTTATTCTCCGCTCTTTCTATTTATCTCCTTCTTTGATACCA
ACATTTTTTGCTTGAAAGAAGTTAATATTTAAGCATTGTTCCGTAGTCTTACTGAAGCTT
TTTCCTCTGTTGTTTTTTGCTATTTTCATTGAGGACTGTG
Primers used for PCR 22af - 8474, show primer details
   
Sequence Name 25-K106287-0022-750-E02C-22af
GenBank Accession LN497456 [GenBank]
Sequencing primer gene specific primer, 22af: 5'-CACAGTCCTCAATGAAAATAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K106287-0022-750-E02C-22af
GAAGCTCGTAGACTACGGAACATGCTTAAATATTAACTTCTTTCAGCAAAAAATGTTGGT
ATCAAAGAAGGAGATAAATAGAAAGAGCGGAGAATAAATAACCAAACTGTAAATTTCTAG
TGAACTTACTTGGCAATGGCTTTCCTTGCTCTCCAGAAACGTTTTTGACCTAATATACCC
ACAACATCATCGGGAGAGACATCTATGCTGGATATAGAATCCACCATCGAATCATCAGAA
ACGTCATCACTTGCCTCTTCATTTATCTGCTGCAGAGAGGCGCATGCTCTGCTTTTGCTG
TATTCACGATCATTATCAATGTCTTCAGGACTGCCATGACCCTCTTGTGAATGATTTTCC
GTTGCCAAGTGAGATTCAGCTCCATTATCAGTGACTTCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTAT
T
   
Sequence Name 25-K106286-0022-750-E02C-8409
GenBank Accession LN497455 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K106286-0022-750-E02C-8409
TTCGGACTGAGCCTTTACATTGAATATATCCTGAAGTCACTGATAATGGAGCTGAATCTC
ACTTGGCAACGGAAAATCATTCACAAGAGGGTCATGGCAGTCCTGAAGACATTGATAATG
ATCGTGAATACAGCAAAAGCAGAGCATGCGCCTCTCTGCAGCAGATAAATGAAGAGGCAA
GTGATGACGTTTCTGATGATTCGATGGTGGATTCTATATCCAGCATAGATGTCTCTCCCG
ATGATGTTGTGGGTATATTAGGTCAAAAACGTTTCTGGAGAGCAAGGAAAGCCATTGCCA
AGTAAGTTCACTAGAAATTTACAGTTTGGTTATTTATTCTCCGCTCTTTCTATTTATCTC
CTTCTTTGATACCAACATTTTTTGCTTGAAAGAAGTTAATATTTAAGCATTGTTCCGTAG
TCTTACTGAAGCTTTTTCCTCTGTTGTTTTTTGCTATTTTCATGANGGACTGTG

Last Updated on 06.12.2018 16:11