Sequences from confirmation amplicon


Confirmation sequences for line 750E08 and hit At1g01050 are shown.
Line ID 750E08
Locus At1g01050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:32944
Primers used for PCR 15nm - 8474, show primer details
   
Sequence Name 54-K114503-0022-750-E08C-15nm
GenBank Accession LN508557 [GenBank]
Sequencing primer gene specific primer, 15nm: 5'-CATATCCTTGCTACCAAACCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K114503-0022-750-E08C-15nm
AGGAGTGACCGCAAAAACACAAACTATGTTTCGTAATAAAAAATAAAGTATTTTTAAAAA
GATGTAAATCTTTGTAAATAATTGAGTGGTAGTGTAGTGAGTGTTACGATAATATAAAAG
TTTGATCCGTATATCTCGGTGGTGACTCATATATACATTTTACAATCACTTGCTAGACCA
ACGGGCTTCACTTGTTTCTCTCCCAAAGTTTCTTCATCATCCTTGCGATAAAGAAAACAA
CAATCGGCTGTTTCGTTTCGATCCAAAGGTACGTTTTTTTTTCTTTTGTCATCGTTCTAA
GATCATCAAAGTTTGATGCTTTTCCATGTTTAATCTTCTGGGTATTTTGTTGCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
TTCCACAACATTATA
   
Sequence Name 51-K114502-0022-750-E08C-8409
GenBank Accession LN507582 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K114502-0022-750-E08C-8409
TGAATATATCCTGCAACAAAATACCCAGAAGATTAAACATGGAAAAGCATCAAACTTTGA
TGATCTTAGAACGATGACAAAAGAAAAAAAAACGTACCTTTGGATCGAAACGAAACAGCC
GATTGTTGTTTTCTTTATCGCAAGGATGATGAAGAAACTTTGGGAGAGAAACAAGTGAAG
CCCGTTGGTCTAGCAAGTGATTGTAAAATGTATATATGAGTCACCACCGAGATATACGGA
TCAAACTTTTATATTATCGTAACACTCACTACACTACCACTCAATTATTTACAAAGATTT
ACATCTTTTTAAAAATACTTTATTTTTTATTACGAAACATAGTTTGTGTTTTTGCGGTCA
CTCCTATAATCTATGTAGGATACTTGCATTACATTTTTCACAAATAATTTGGTTTGGTAG
CAAGGATATG

Last Updated on 06.12.2018 16:11