Sequences from confirmation amplicon


Confirmation sequences for line 750F07 and hit At5g48530 are shown.
Line ID 750F07
Locus At5g48530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19668196
Primers used for PCR 93rq - 8474, show primer details
   
Sequence Name 65-K116204-0022-750-F07C-93rq
GenBank Accession LN511472 [GenBank]
Sequencing primer gene specific primer, 93rq: 5'-CCACTCAAGAAGAAAGAGGAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K116204-0022-750-F07C-93rq
GTACCTCTGCGTGAGCGTTTCACAATCCCGAGAGACAATATCCCCAATAAGCGCCGGAAA
CAGCTCCGTGAGCAGTTCATGCGACGAACTCGTCTCGTTCTTAAAGAATCGGTCAGTGTC
TTTTCACAATGTTAGGATTTAGAGGACTGAATATGAATGTGTAGCTTTTGATTACTGATT
GGATTAGTATGATTAGGAGCATGAGCCATGGTGCAAGAAGTACATGGAATTGTATAATGA
GGTTAGAGAAAACTGGGAGAGGCTTTATTGGGATGAAGGATATTCTAAGAAAATTGCGAG
AGATCATGCTAATTATGAGTCTGCTGAAGAAGATGATGAAGATTTTAATCCATACAGGTT
TCTTTATTTGTGGAGATAGCAATGTTACAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 29-K116203-0022-750-F07C-8409
GenBank Accession LN499438 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K116203-0022-750-F07C-8409
ATTTACATTGATATATCCTGTAACATTGCTATCTCCACAAATAAAGAAACCTGTATGGAT
TAAAATCTTCATCATCTTCTTCAGCAGACTCATAATTAGCATGATCTCTCGCAATTTTCT
TAGAATATCCTTCATCCCAATAAAGCCTCTCCCAGTTTTCTCTAACCTCATTATACAATT
CCATGTACTTCTTGCACCATGGCTCATGCTCCTAATCATACTAATCCAATCAGTAATCAA
AAGCTACACATTCATATTCAGTCCTCTAAATCCTAACATTGTGAAAAGACACTGACCGAT
TCTTTAAGAACGAGACGAGTTCGTCGCATGAACTGCTCACGGAGCTGTTTCCGGCGCTTA
TTGGGGATATTGTCTCTCGGGATTGTGAAACGCTCACGCAGAGGTACGTGATCTCCGATC
TCGTGCATCTCTCCGTCGACGATCCACCACTCTGATTTCTCCTCTTTTTCCTTGGAGTGG

Last Updated on 06.12.2018 16:11