Sequences from confirmation amplicon


Confirmation sequences for line 750G07 and hit At3g47060 are shown.
Line ID 750G07
Locus At3g47060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17335421
Primers used for PCR 25ey - 8474, show primer details
   
Sequence Name 57-K111119-0022-750-G07C-25ey
GenBank Accession LN509452 [GenBank]
Sequencing primer gene specific primer, 25ey: 5'-GCTTAATTTCTGACAACACCACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K111119-0022-750-G07C-25ey
TTGCACGTACTAACAACATAAACAATGAGCGTAAATGTAGACAAAGTTTTTGCATTTTAC
CTCAACACGTGGCTGTCCAGTAAGAAGATTTGCAACAGCGGTACCAACCACTGCATGCCC
AGCTTCATGCCTTGCGACCACTGCCTTCTCATTGCCTTTTAATCTTGCAGATTTCTTCTC
TATGCCCTGAAACAGGTCTTTGTAAGAAAACACATTTTGACAATTGCCTTACGTAATCAA
TAAAACAGCGAGGTTATAGAGATACCGCTATTGATCGCTCCACAGCTTGAATGAAGTCAA
TTTTCTCAACGTTCGTCTTGTTCTTTCTTCCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 41-K111118-0022-750-G07C-8409
GenBank Accession LN504117 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K111118-0022-750-G07C-8409
CATTGAATATTCCTGGAAGAAAGAACAAGACGAACGTTGAGAAAATTGACTTCATTCAAG
CTGTGGAGCGATCAATAGCGGTATCTCTATAACCTCGCTGTTTTATTGATTACGTAAGGC
AATTGTCAAAATGTGTTTTCTTACAAAGACCTGTTTCAGGGCATAGAGAAGAAATCTGCA
AGATTAAAAGGCAATGAGAAGGCAGTGGTCGCAAGGCATGAAGCTGGGCATGCAGTGGTT
GGTACCGCTGTTGCAAATCTTCTTACTGGACAGCCACGTGTTGAGGTAAAATGCAAAAAC
TTTGTCTACATTTACGCTCATTGTTTATGTTGTTTAGTACGTGCAAATGATTCAAGTTTT
GCTCATCTTTGTGGTGTTGTCAANAATTAAG

Last Updated on 06.12.2018 16:11