Sequences from confirmation amplicon


Confirmation sequences for line 751A12 and hit At2g32670 are shown.
Line ID 751A12
Locus At2g32670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13858094
Primers used for PCR BE33 - 8474, show primer details
   
Sequence Name 48-K104356-0022-751-A12C-BE33
GenBank Accession LN506499 [GenBank]
Sequencing primer gene specific primer, BE33: 5'-CCTTACCACCACCATATCTCTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K104356-0022-751-A12C-BE33
CAGAAGCATAGGAATCTGCCTCCAACAGATTCAACAGCAACAACACAATACGCTGCATAA
CAACAATTTTATCAGATCCATCAAAGCAATTATTACTTCATTGCCAAAAGTAGATATAGC
AATGCAACTTCTGAATTCCATCATTGCAAAAAAAAAAAATAGAGATCAAGATAGACTTTG
TTTAAACCAAAACTTATGGTAACGCGGGTTTCAGGCTTAAAGAGAATCATATATGGAGAT
CGAAAAAAAAAAACTCACTGAACCCTTTCTCAACAAGGTAATTGAACGTGGGTCCGTCCC
ATTTGTATGTAAACTTGTTATTCAAAAAAGGAAGCTTTTGAAAACACTGTGCACCGACGG
CGGTGAAATTTCCTTTGAATTCGGTATACCCAACAAGAATCCCCGTACCACGAGCGACAA
ACCTGTAAATCAAATTTTGTTGTCCCATTGTTCTCGGGACCCGAAAAGGGAAAAAAAAAA
AAGAGTATTTCCCATTTTTTTTGAATATTTCCAATTGTAAAGGGCTTCAGGTCCGGGAAA
TCTACTGGGATCACCAATGAGTATGAGGGTCAATATGGAAAAAAAAAAAGAGTAATTACC
AATTTTTTTTCATTTCAAAAATGTAAATTTC
   
Sequence Name 64-K104357-0022-751-A12C-8409
GenBank Accession LN511196 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K104357-0022-751-A12C-8409
ATTTACATTGAATATATTCAAAAAAATTGGTAATTACTCTTTCTTTTTCTTCCTTCTTCG
GGTCACGAGAACAATGGGACAACAAAATTTGATCTACAGCTTCGTCGCTCGTGGTACGGT
GATTCTCGTCGAGTATACAGAATTCAAAGGAAATTTCACTGCCGTCGCTGCACAGTGTCT
CCAAAAGCTTCCTTCTTCGAATAACAAGTTTACATACAATTGCGACGGACACACGTTCAA
TTACCTCGTCGAGAATGGCTTCAGTGAGTCTCTTCTTCTCGATCTCTATATATGATTCTC
TCTAAGCTTGAAACTCGCGTTACAATAAGTTTCGGTTTAATCAATGTCTATCTTGATCTC
TATTTTTTTTTTTTGCAAGGAGGGAATTCAAAATTTGCATTGCTATATCTACTTTGGGCA
ATAAAGAAAAAATTGCTTTGAGGGATCTAAAAAAATTGTTTTTATGCACCGAATTGGGTT
GTTGCTGTTAAATCTTTGGGGAGGCAAATTCCTATGGTTTTCTGGGAAAAATTTAAGGAA
AATTTCAACAAAAAATATGGG
Primers used for PCR CB32 - 3144, show primer details
   
Sequence Name 68-K104767-0022-751-A12C-CB32
GenBank Accession LN512011 [GenBank]
Sequencing primer gene specific primer, CB32: 5'-TTCAAACTTTAAGCATCCACACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K104767-0022-751-A12C-CB32
ACAAAGCGAAAATTTCTGATTGTTTCATATTTAACAACCTTAAAATTCATGAACCATTAT
TCCGAAACTGGTACTTTTAGAAAACAGAAACATTAAATAGTTAAAGTAATTAACTAGAAT
TAAATAACGCAATTAATTTTTGAAAAAAAAACGTTGATAGAAAGGAGAAAAATGGATCGC
AGCGTCGTCCCTATATCTCTCGCTCCCTTTCAATTTCTCCTTGTATTTTGGATCTTTCTT
ACTTCCGTACACACAAACCCAAACAAACAACAAAAAACTGTTGTCTCTCTCTCTCTTTGG
TGGAATTCAAAAAATCGAATTCGTGAAAAGAAAGAAATGAAATGAACTTCCTTATATAGA
GGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATC
ACATCAATCC
   
Sequence Name 68-K104768-0022-751-A12C-3144
GenBank Accession LN512012 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K104768-0022-751-A12C-3144
TCCACATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTCTTTCTTTTC
ACGAATTCGATTTTTTGAATTCCACCAAAGAGAGAGAGAGACAACAGTTTTTTGTTGTTT
GTTTGGGTTTGTGTGTACGGAAGTAAGAAAGATCCAAAATACAAGGAGAAATTGAAAGGG
AGCGAGAGATATAGGGACGACGCTGCGATCCATTTTTCTCCTTTCTATCAACGTTTTTTT
TTCAAAAATTAATTGCGTTATTTAATTCTAGTTAATTACTTTAACTATTTAATGTTTCTG
TTTTCTAAAAGTACCAGTTTCGGAATAATGGTTCATGAATTTTAAGGTTGTTAAATATGA
AACAATCAGAAATTTTTCGCTATTTGTTTATATGTAACCATATATAGCAGTGTGGATGCT
TAAAGTTTGA

Last Updated on 06.12.2018 16:11