Sequences from confirmation amplicon


Confirmation sequences for line 751C01 and hit At4g26060 are shown.
Line ID 751C01
Locus At4g26060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13216078
Primers used for PCR NL66 - 8474, show primer details
   
Sequence Name 38-K104195-0022-751-C01C-NL66
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, NL66: 5'-AGAAAAGTCCATTCATTTTTCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K104195-0022-751-C01C-NL66
CAAAATTATGTGAGTAAAACCTTCTTTATGGATTACTTAATATAATTCAGGAAAGGGACA
AAAAATTAGGGGAAAAATTAAATACCCGTAAAGTTGTTAAAACTTTAAAAGTTTTTAATT
AACCAAAATATTACTAAAAAAGAAACAAACTTGCTTTAGGCAAAAAAAAAAAAAAACTTT
CTTTTTTCTGGCACCTCCCGCTCTCTACCCATTTATCACCCTAACAAATTTAAAACAGCC
ACCACAAAAAAAAAACAAACCACAACAACCCTTTCGCTCCCAAATTTCTGGACCCAATTT
TTATTTCCCCCCGCCCCTGGAAATCAAATCTCTCCCCAAATTTGGGCCCTAAAATTTATA
ACCGGGTAAGTTATTCACCCCCTTCGGTTCCCTTTTACTTCCCGGACCATTCCCCGGTTC
GGGGGGATTTCATTCTCCCAATAAATTTAAAGCCCTATGGCCCCAAATATTTCCTTTTCC
CAAATAAAGAAAATAACCTAATTCAAACTTTTTCAGGCTCCTTTAAAGGGCTCCAGGTCC
GGGAAATCTCCTGGGATCACCATTGAGTATAAGGGTCAATATGGAAAAAAAAAAAGAGTA
TTTCCCATTTTTTTTTCATTTCAAAAATGTAAATTTCCC
   
Sequence Name 54-K104196-0022-751-C01C-8409
GenBank Accession LN508465 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K104196-0022-751-C01C-8409
TTCGGACTGAGCCTTACAGAAGCATGAAAAAGTCTGAATCAGATTATCTTCTCTATTTGG
AAAAAGAACATATATGAGACAATAGGACTTCAAATTCATCGGAAGAATGAAATCCACACG
AACCGGAGACTGCTCCGGGAAGTAAAAGGAAACCGATGGAGATGAATAACTTACCAGATT
ATAATTTCTAGAGCACAAATTCGAGAAGAGATTTGATTTCAAGGGCAGAGGGAATATAAA
TATCGGATCAAGAAATCTGGAAGCGAGAGAGTCGTCGTGCTTCGTCTTCTTCTTGTGCTG
CTTGTTTCAACTTCGTCAGCGTGATAAATGCGTAGAGAGCGGAAGATGCAAGAAAAATGA
AAGTCTATTTTTTCTTTTGCCTAAAGCAAGTTTGTCTCTTTTCTAGTACTATTTTGCTTA
ATTAAATACTTTTAATGTCTTAACAACTATACGAGTATTTAATTCTTCCACTAATCTTTT
GTCCTTTTCATGATTTATATTATGTAATCAATAATGATGTTTTTACTCACATAATTTTGT
AGTGAAATTAGCTCTAAGAAATTACAATTTTTCTATTTCTTTTTTTTTTTTGGAAAAT
Primers used for PCR 03rg - 8474, show primer details
   
Sequence Name 28-K118380-0022-751-C01C-03rg
GenBank Accession LN499045 [GenBank]
Sequencing primer gene specific primer, 03rg: 5'-TGTTATCTCATCTCAAGACCATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118380-0022-751-C01C-03rg
AACAATCACTTCCAGCTAGTCAACAACTTAGTTGTGAGAATTTAGATGATCCTCTATTCT
AGTAACAAGAACTAACAAAGCACATATCTTTTGAGGACTAATATGAATCGAAAGAGATAA
AAAGGGAGGAAGATGATTACTTTCTCCCATACGTTCTTCAGCAGATGATGAAAATGCTAA
GCTCTTGCTTATAAGTTTGTGTCGTGACCCACAAGATCTTCAAATTGCCTGCAACAAAAC
ATTAACAATATGAATGAATTTCAGAAAGGAAAACATAGAAAACAGAGACGATTTCAGCTA
AAACCGTAGCAGCAATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTC
   
Sequence Name 18-K118379-0022-751-C01C-8409
GenBank Accession LN494510 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118379-0022-751-C01C-8409
TTTTCCTTTCTGAAATTCATTCATATTGTTAATGTTTTGTTGCAGGCAATTTGAAGATCT
TGTGGGTCACGACACAAACTTATAAGCAAGAGCTTAGCATTTTCATCATCTGCTGAAGAA
CGTATGGGAGAAAGTAATCATCTTCCTCCCTTTTTATCTCTTTCGATTCATATTAGTCCT
CAAAAGATATGTGCTTTGTTAGTTCTTGTTACTAGAATAGAGGATCATCTAAATCTCACA
ACTAAGTTGTTGACTAGCTGGAAGTGATTGTTCACCAACAGTCAAAGATCATAAATTTTT
GTTTTAGGAACAAGGGGGCATGGTCTTGAATAAAAAATAACA

Last Updated on 06.12.2018 16:11