Sequences from confirmation amplicon


Confirmation sequences for line 751C09 and hit At4g33490 are shown.
Line ID 751C09
Locus At4g33490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16110447
Primers used for PCR NL67 - 8474, show primer details
   
Sequence Name 10-K103785-0022-751-C09C-NL67
GenBank Accession LN490621 [GenBank]
Sequencing primer gene specific primer, NL67: 5'-TTTTCAAACTTCAACATCAGCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K103785-0022-751-C09C-NL67
AAATAATTTTCTCTTATTTATTATTTTGCCACAGCCGTGAAAAGATGGAGAAAATGAATG
TCCGTTTTATGATTGTGTTGATGGTAATGTCTCTGGTTCTGGGTTTTTCGTCGGCAGTTG
ATTTCAGATGGAGGAAAACTGCAGGATTCTCAGATAGATTCACCAGAGCTGTTTCTTCAG
TCGTGTTCCCAGTTCATGGCAACGTTTATCCTCTTGGGTAATCTATATATTTGCAAACTC
AACTTCACAAAATTATTGCGTGCGTTCCCTCATGTTCATGTTCTCACTGAAATTCATTTA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATT
   
Sequence Name 26-K103786-0022-751-C09C-8409
GenBank Accession LN497890 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K103786-0022-751-C09C-8409
CATTTACATTGAATATAAATGAATTTCAGTGAGAACATGAACATGAGGGAACGCACGCAA
TAATTTTGTGAAGTTGAGTTTGCAAATATATAGATTACCCAAGAGGATAAACGTTGCCAT
GAACTGGGAACACGACTGAAGAAACAGCTCTGGTGAATCTATCTGAGAATCCTGCAGTTT
TCCTCCATCTGAAATCAACTGCCGACGAAAAACCCAGAACCAGAGACATTACCATCAACA
CAATCATAAAACGGACATTCATTTTCTCCATCTTTTCACGGCTTGTGGCAAAATAATAAA
ATAAAGAGAAAATTTTATTTGTATTAGCCAATCACGTGATGCTGATGTTGAAGTTTGAAA

Last Updated on 06.12.2018 16:11